Evidence for the transcriptional inhibition by heme of the synthesis of δ-aminolevulinate synthase in rat liver
| Autor: | Norio Hayashi, Goro Kikuchi, Masayuki Yamamoto |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Reticulocytes Lysis Transcription Genetic Biophysics Heme Biochemistry chemistry.chemical_compound Reticulocyte Polysome Acetamides medicine Animals RNA Messenger Molecular Biology Messenger RNA ATP synthase biology Rats Inbred Strains Cell Biology Molecular biology In vitro Rats Kinetics medicine.anatomical_structure Liver chemistry Polyribosomes biology.protein Allylisopropylacetamide Rabbits 5-Aminolevulinate Synthetase Hemin |
| Zdroj: | Biochemical and Biophysical Research Communications. 105:985-990 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(82)91067-1 |
| Popis: | Intravenous administration of various doses of hemin to allylisopropylacetamide-pretreated rats resulted in a dose-dependent and time-dependent decrease of the ability of liver polysomes to direct the synthesis of δ-aminolevulinate synthase in vitro in a reticulocyte lysate system. The apparent half-time of decrease of the ability of polysomes observed at a maximal dose of hemin was about 60 min; this value is comparable to the previously estimated half-life of δ-aminolevulinate synthase mRNA. It was assumed that heme inhibits the synthesis of δ-aminolevulinate synthase also at a transcriptional step. δ-Aminolevulinate synthase synthesized in vitro had a minimum molecular weight of about 75,000, whereas the value for the liver mitochondrial δ-aminolevulinate synthase was about 66,000. |
| Databáze: | OpenAIRE |
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