Identification and prevalence of RND family multidrug efflux pump oqxAB genes in Enterococci isolates from swine manure in China
| Autor: | Hua-Run Sun, Gong-Zheng Hu, Yu-Shan Pan, Ya-bin Wang, Nan-nan Kuang, Hua Wu, Li Yuan, Ya-Jun Zhai, Zhi-pei He |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Florfenicol China Sequence analysis Swine 030106 microbiology Microbial Sensitivity Tests Quinolones Microbiology Polymerase Chain Reaction Enterococcus faecalis 03 medical and health sciences chemistry.chemical_compound Quinoxalines Drug Resistance Bacterial Operon medicine Escherichia coli Animals Enrofloxacin biology High-Throughput Nucleotide Sequencing Membrane Transport Proteins Kanamycin General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents Ciprofloxacin Manure Enterococcus chemistry Conjugation Genetic Efflux Cell Division medicine.drug Fluoroquinolones Plasmids |
| Zdroj: | Journal of medical microbiology. 67(6) |
| ISSN: | 1473-5644 |
| Popis: | Purpose. The resistance/nodulation/cell division (RND) family multidrug efflux pump, OqxAB, has been identified as one of the leading mechanisms of plasmid-mediated quinolone resistance and has become increasingly prevalent among Enterobacteriaceae in recent years. However, oqxAB genes have not yet been reported in Enterococcus isolates. The aim of the present study was to identify the oqxAB genes and investigate their prevalence among Enterococcus from swine manure in China. Methodology. The oqxAB genes were screened in 87 Enterococcus isolates by PCR. The transferability of the oqxAB genes in Enterococcus was determined by conjugation experiments. The genetic environment of oqxAB genes was investigated by cloning experiments, PCR mapping and sequencing. Results. A high prevalence (86.2 %) of olaquindox resistance was observed in Enterococcus and 98.9 % isolates exhibited multidrug-resistance phenotypes. The occurrence of oqxA and oqxB in Enterococcus was also high (79.3 and 65.5 %, respectively). Sequence analysis of the cloned fragment indicated that the oqxAB cassette was linked to an incomplete Tn5 transposon containing aph(3′)-IIa and flanked by IS26 [IS26-oqxAB-IS26-aph(3′)-IIa]. The oqxAB–aph(3′)-IIa-positive transconjugant or transformant showed resistance or reduced susceptibility to enrofloxacin, ciprofloxacin, olaquindox, mequindox, florfenicol, neomycin and kanamycin. Conclusion. This is the first time that the oqxAB genes have been identified in Enterococcus faecalis from swine manure. The genetic linkage of oqxAB–aph(3′)-IIa in Enterococcus has not been described before. The high prevalence of oqxAB genes in Enterococcus suggests that it may constitute a reservoir for oqxAB genes and pose a potential threat to public health. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|