Isolation and Histopathological Changes Associated with Non-Tuberculous Mycobacteria in Lymph Nodes Condemned at a Bovine Slaughterhouse
| Autor: | Gloria María Molina-Salinas, José L. Palomares-Rangel, Ned I. de la Cruz-Hernandez, Julio Martínez-Burnes, Angélica M Hernández-Jarguín, Alfonso López Mayagoitia, Hugo Barrios-García |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Tuberculosis 040301 veterinary sciences Tuberculin granulomatous lesions Article Microbiology 0403 veterinary science non-tuberculous mycobacteria 03 medical and health sciences medicine Tuberculin test tuberculin test Mycobacterium bovis lcsh:Veterinary medicine General Veterinary biology 04 agricultural and veterinary sciences bacterial infections and mycoses biology.organism_classification Isolation (microbiology) medicine.disease Confounding effect 030104 developmental biology tuberculosis lcsh:SF600-1100 Histopathology Lymph |
| Zdroj: | Veterinary Sciences Veterinary Sciences, Vol 7, Iss 172, p 172 (2020) Volume 7 Issue 4 |
| ISSN: | 2306-7381 |
| Popis: | Background: non-tuberculous mycobacteria (NTM) infect humans and animals and have a critical confounding effect on the diagnosis of bovine tuberculosis. The Official Mexican Standard (Norma Oficial Mexicana, NOM-ZOO-031-1995) for food safety regulates Mycobacterium bovis in cattle, but not the NTM species. The study&rsquo s objective was to isolate and identify the NTM present in condemned bovine lymph nodes in a slaughterhouse, characterize the histological lesions, and correlate bacteriological and microscopic findings with the antemortem tuberculin skin test. Methods: from 528 cattle, one or two pooled samples of lymph nodes from each animal were cultured for Mycobacteria spp. and processed for histopathology. Results: mycobacteria were isolated from 54/528 (10.2%) of the condemned lymph nodes 25/54 (46.2%) of these isolates were NTM 4 bacteriological cultures with fungal contamination were discarded. Granulomatous and pyogranulomatous inflammation were present in 6/21 (28.6%) and 7/21 (33.3%) of the NTM-positive lymph nodes, respectively. The species of NTM associated with granulomatous lymphadenitis were M. scrofulaceum, M. triviale, M. terrae, and M. szulgai, while those causing pyogranulomatous lesions were M. szulgai, M. kansasii, M. phlei, and M. scrofulaceum. Conclusions: the NTM infections can cause false-positive results in the tuberculin test because of cross immune reactivity and interference with the postmortem identification of M. bovis in cattle. |
| Databáze: | OpenAIRE |
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