Application of ES cells for generation of respiration-deficient mice carrying mtDNA with a large-scale deletion
| Autor: | Naoki Watanabe, Atsuko Kasahara, Yoko Suda, Akitsugu Sato, Kaori Ishikawa, Jun-Ichi Hayashi, Shinichi Aizawa, Kazuto Nakada |
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| Rok vydání: | 2005 |
| Předmět: |
Mitochondrial DNA
Mitochondrial Diseases Transgene Biophysics Mice Transgenic Biology Kidney DNA Mitochondrial Biochemistry Mice chemistry.chemical_compound Chimera (genetics) Animals Molecular Biology Cells Cultured Mice Inbred ICR Zygote Stem Cells Gene Transfer Techniques Kidney metabolism Cell Biology Respiration Disorders Embryonic stem cell Molecular biology Mitochondria Disease Models Animal chemistry Female Stem cell DNA |
| Zdroj: | Biochemical and Biophysical Research Communications. 333:590-595 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2005.05.155 |
| Popis: | In a previous study, we used mouse zygotes as recipients of mtDNA with a large-scale deletion mutation (DeltamtDNA) and generated respiration-deficient mice (mito-mice) carrying DeltamtDNA. In this study, we used mouse ES cells as recipients of DeltamtDNA, and generated mito-mice with DeltamtDNA only when the ES cells carried 17% DeltamtDNA. No chimera mice or their F(1) progenies were obtained from ES cells carrying more than 61% DeltamtDNA. These observations suggest that respiratory defects of ES cells inhibit their normal differentiation into chimera mice and mito-mice, and that ES cells are more effective than zygotes for generation of mito-mice carrying mtDNAs without significant pathogenic mutations. |
| Databáze: | OpenAIRE |
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