A Portable Raman Acoustic Levitation Spectroscopic System for the Identification and Environmental Monitoring of Algal Cells
| Autor: | John Beardall, Donald McNaughton, Danielle Morrison, Bayden R. Wood, Slobodanka Stojkovic, Philip Heraud |
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| Rok vydání: | 2005 |
| Předmět: |
Chlorophyll a
Nitrogen Pheophytins Analytical chemistry Spectrum Analysis Raman Acoustic levitation Spectral line Analytical Chemistry law.invention chemistry.chemical_compound symbols.namesake Halogen lamp chemistry Chlorophyta law Chlorophyll Environmental monitoring symbols Raman spectroscopy Quartz Environmental Monitoring |
| Zdroj: | Analytical Chemistry. 77:4955-4961 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | We report the coupling of a portable Raman spectrometer to an acoustic levitation device to enable environmental monitoring and the potential taxonomic identification of microalgae. Spectra of living cells were recorded at 785 nm using a fiber-optic probe coupled to a portable Raman spectrometer. The spectra exhibit an excellent signal-to-noise ratio and clearly show bands from chlorophyll a and beta-carotene. Spectra of levitated photobleached microalgae clearly show a reduction in chlorophyll a concentration relative to beta-carotene after 10 min of exposure to a quartz halogen lamp. Spectra recorded from levitated nitrogen-limited cells also show a significant reduction in bands associated with chlorophyll a, as compared to nitrogen-replete cells. To investigate the diagnostic capability of the technique, four species of microalgae were analyzed. Good quality spectra of all four species were obtained showing varying ratios of beta-carotene to chlorophyll. The combination of an acoustic levitation device and a portable Raman spectrometer shows potential as a taxonomic and environmental monitoring tool with direct application to field studies in remote environments. |
| Databáze: | OpenAIRE |
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