The increased motion of lumbar induces ligamentum flavum hypertrophy in a rat model
| Autor: | Jingru Zhang, Chun-Yu Gao, Baojian Wang, Ping Zhang, Jinghua Gao, Wu Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Pathology lcsh:Diseases of the musculoskeletal system Increased motion Rat model H&E stain Fibrotic factor Muscle hypertrophy Masson's trichrome stain 03 medical and health sciences Masson trichrome staining 0302 clinical medicine Lumbar Spinal Stenosis Rheumatology Internal medicine medicine Animals Orthopedics and Sports Medicine Inflammatory factor 030304 developmental biology 0303 health sciences Lumbar Vertebrae Experimental study business.industry Lumbosacral Region Hypertrophy Immunohistochemistry Staining Rats Lumbar degenerative disease Ligamentum flavum Real‐time PCR lcsh:RC925-935 business 030217 neurology & neurosurgery Research Article |
| Zdroj: | BMC Musculoskeletal Disorders BMC Musculoskeletal Disorders, Vol 22, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1471-2474 |
| Popis: | Background The purpose of this study was to establish a novel rat model for ligamentum flavum (LF) hypertrophy using increased motion of lumbar and to elucidate the etiology of (LFH). Methods A total number of 30 male rats were used. The increased motion of lumbar was induced by surgical resection of L5/6 posterior elements (n = 15). The other rats underwent a sham operation (n = 15). After 8 weeks, all rats were taken lateral plain X-rays. The LF from L5/6 in both groups were harvested to investigate histological, immunohistological, and real-time PCR analysis. Results According to radiological results, the disc height ratio, flexion ratio, and extension ratio were larger in the rats in the experimental group than that of in the sham group. The HE staining showed that the LF thickness in the experimental group significantly increased in comparison to the sham group. The Masson trichrome staining showed that the ratio of elastic fibers to collagen fibers in experimental group was lower than that in the sham group. The protein and gene expression of TGF-β1, TNF-α, IL-1β, and Col 1 were significantly higher in the experimental group than that in the sham group. Conclusion A relatively safe, simple, and rapid rat model of LFH using increased motion of lumbar was established. The increased motion of lumbar could lead to high expression of inflammatory and fibrotic factors in LF, causing the accumulation of collagen fibers and decreasing of elastic fibers. |
| Databáze: | OpenAIRE |
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