Production of interleukin-1 by bone marrow myeloma cells
| Autor: | A R Shaw, P M Lansdorp, F. Cozzolino, A Miliani, R Di Guglielmo, Donatella Aldinucci, Maria Torcia, A. Rubartelli |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
biology
business.industry medicine.medical_treatment Immunology Interleukin Cell Biology Hematology Plasma cell medicine.disease Molecular biology Biochemistry Cytokine medicine.anatomical_structure Cell culture medicine biology.protein Tumor necrosis factor alpha Bone marrow Antibody business Monoclonal gammopathy of undetermined significance |
| Zdroj: | Scopus-Elsevier Università degli studi di Firenze-IRIS |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v74.1.380.bloodjournal741380 |
| Popis: | Plasma cells isolated from bone marrow (BM) aspirates of 12 patients with multiple myeloma (MM) and nine patients with monoclonal gammopathy of undetermined significance (MGUS) were analyzed for production of cytokines with bone-resorbing activity, such as interleukin-1 (IL-1), tumor necrosis factor (TNF), and lymphotoxin (LT). Culture supernatants of plasma cells from MM, but not from MGUS or normal donor, invariably contained high amounts of IL-1-beta and lower amounts of IL-1-alpha. With a single exception, TNF/LT biologic activity was not detected in the same supernatants. IL-6 was present in two of five supernatants tested. Normal B lymphocytes released both IL-1 and TNF/LT activities for four days after activation in vitro; however, production of these cytokines ceased at the final stage of plasma cell. Unexpectedly, the mRNA extracted from MM plasma cell hybridized with TNF- and LT- specific, as well as IL-1-specific probes, although the culture supernatants did not contain detectable TNF/LT biologic activity. When tested in the fetal rat long bone assay, MM plasma cell supernatants displayed a strong osteoclast-activating factor (OAF) activity, which was greatly reduced but not completely abolished by neutralizing anti- IL-1 antibodies. Anti-TNF or anti-LT antibodies were ineffective in the same test. We conclude that the IL-1 released in vivo by malignant plasma cells has a major role in pathogenesis of lytic bone lesions of human MM. |
| Databáze: | OpenAIRE |
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