T cell receptor recognition of a 'super-bulged' major histocompatibility complex class I-bound peptide
| Autor: | John J. Miles, Travis Clarke Beddoe, Scott R. Burrows, Lars Kjer-Nielsen, Jamie Rossjohn, James C. Whisstock, Fleur Elizabeth Tynan, Jacqueline M. Burrows, Anthony W. Purcell, Lyudmila Kostenko, Sharon L. Silins, James McCluskey, Ashley M. Buckle, Craig Steven Clements, Matthew C.J. Wilce, Natalie A. Borg |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Protein Conformation
Receptors Antigen T-Cell alpha-beta Immunology Molecular Sequence Data Epitopes T-Lymphocyte chemical and pharmacologic phenomena Peptide Major histocompatibility complex Crystallography X-Ray Epitope Cell Line Immune system MHC class I Immunology and Allergy Humans Amino Acid Sequence Genetics chemistry.chemical_classification Antigen Presentation biology T-cell receptor hemic and immune systems MHC restriction Cell biology chemistry HLA-B Antigens biology.protein Peptides CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Nature immunology. 6(11) |
| ISSN: | 1529-2908 |
| Popis: | Unusually long major histocompatibility complex (MHC) class I-restricted epitopes are important in immunity, but their 'bulged' conformation represents a potential obstacle to alphabeta T cell receptor (TCR)-MHC class I docking. To elucidate how such recognition is achieved while still preserving MHC restriction, we have determined here the structure of a TCR in complex with HLA-B(*)3508 presenting a peptide 13 amino acids in length. This complex was atypical of TCR-peptide-MHC class I interactions, being dominated at the interface by peptide-mediated interactions. The TCR assumed two distinct orientations, swiveling on top of the centrally bulged, rigid peptide such that only limited contacts were made with MHC class I. Although the TCR-peptide recognition resembled an antibody-antigen interaction, the TCR-MHC class I contacts defined a minimal 'generic footprint' of MHC-restriction. Thus our findings simultaneously demonstrate the considerable adaptability of the TCR and the 'shape' of MHC restriction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|