Stabilization and Localization of Xist RNA are Controlled by Separate Mechanisms and are Not Sufficient for X Inactivation
| Autor: | Jennifer C. Chow, Carolyn J. Brown, Christine Moulton Clemson, Jeanne B. Lawrence |
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| Rok vydání: | 1998 |
| Předmět: |
RNA
Untranslated X Chromosome RNA localization Hybrid Cells Biology X-inactivation Cell Line Mice 03 medical and health sciences 0302 clinical medicine Dosage Compensation Genetic medicine Animals Humans RNA Messenger In Situ Hybridization Fluorescence X chromosome 030304 developmental biology Cell Nucleus 0303 health sciences RNA Articles Cell Biology Non-coding RNA Molecular biology Chromatin Cell nucleus medicine.anatomical_structure RNA Long Noncoding XIST 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.142.1.13 |
| Popis: | These studies address whether XIST RNA is properly localized to the X chromosome in somatic cells where human XIST expression is reactivated, but fails to result in X inactivation (Tinker, A.V., and C.J. Brown. 1998. Nucl. Acids Res. 26:2935–2940). Despite a nuclear RNA accumulation of normal abundance and stability, XIST RNA does not localize in reactivants or in naturally inactive human X chromosomes in mouse/ human hybrid cells. The XIST transcripts are fully stabilized despite their inability to localize, and hence XIST RNA localization can be uncoupled from stabilization, indicating that these are separate steps controlled by distinct mechanisms. Mouse Xist RNA tightly localized to an active X chromosome, demonstrating for the first time that the active X chromosome in somatic cells is competent to associate with Xist RNA. These results imply that species-specific factors, present even in mature, somatic cells that do not normally express Xist, are necessary for localization. When Xist RNA is properly localized to an active mouse X chromosome, X inactivation does not result. Therefore, there is not a strict correlation between Xist localization and chromatin inactivation. Moreover, expression, stabilization, and localization of Xist RNA are not sufficient for X inactivation. We hypothesize that chromosomal association of XIST RNA may initiate subsequent developmental events required to enact transcriptional silencing. |
| Databáze: | OpenAIRE |
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