Microinjected c- myc as a Competence Factor
| Autor: | Rosemary Watt, Martin Rosenberg, Renato Baserga, Julia K. Hyland, Leszek Kaczmarek |
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| Rok vydání: | 1985 |
| Předmět: |
Platelet-Derived Growth Factor
Multidisciplinary DNA synthesis Cell division Cell growth Growth factor medicine.medical_treatment Cell Cycle Cell DNA Oncogenes Cell cycle Biology Molecular biology Mice medicine.anatomical_structure medicine biology.protein Animals Cells Cultured Intracellular Platelet-derived growth factor receptor |
| Zdroj: | Science. 228:1313-1315 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.4001943 |
| Popis: | While a number of oncogenes are expressed in a cell cycle-dependent manner, their role in the control of cell proliferation can only be established by a direct functional assay. The c-myc protein, upon microinjection into nuclei of quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma in the stimulation of cellular DNA synthesis. This suggests that c-myc protein, like platelet-derived growth factor (PDGF), may act as a competence factor in the cell cycle to promote the progression of cells to S phase. The presence in the medium of an antibody against PDGF abolished DNA synthesis induced by microinjected PDGF; however, the microinjected c-myc protein stimulated DNA synthesis even when its own antibody was present in the medium. The c-myc protein may act as an intracellular competence factor, while PDGF expresses its biological activity only from outside the cells. |
| Databáze: | OpenAIRE |
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