tRNA 2'-O-methylation by a duo of TRM7/FTSJ1 proteins modulates small RNA silencing in Drosophila
| Autor: | Jean-Yves Roignant, Margarita T Angelova, Vincent Guérineau, Caroline Jacquier, Clément Carré, Bruno Da Silva, Christophe Antoniewski, Matthias Schaefer, Souraya Khouider, Mira Brazane, Yuri Motorin, Salman Shehzada, Damien Brégeon, Tina Lence, Virginie Marchand, Valérie Bourguignon-Igel, Dilyana G. Dimitrova, Cyrinne Achour, Catherine Goyenvalle, Laure Teysset |
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| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ingénierie, Biologie et Santé en Lorraine (IBSLor), Université de Lorraine (UL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institute of Molecular Biology (IMB), Johannes Gutenberg - Universität Mainz (JGU), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Center for Integrative Genomics - Institute of Bioinformatics, Génopode (CIG), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL)-Université de Lausanne (UNIL), Medizinische Universität Wien = Medical University of Vienna |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Small RNA
Saccharomyces cerevisiae Proteins Animals Drosophila melanogaster/genetics Gene Expression Regulation/genetics Gene Silencing Humans Methylation Methyltransferases/genetics Nuclear Proteins/genetics RNA Interference RNA Transfer/genetics Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization tRNA Methyltransferases/genetics Saccharomyces cerevisiae Biology 03 medical and health sciences RNA Transfer RNA interference [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Genetics Gene 030304 developmental biology 0303 health sciences tRNA Methyltransferases 2'-O-methylation Nucleic Acid Enzymes 030302 biochemistry & molecular biology RNA Nuclear Proteins Translation (biology) [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Methyltransferases Cell biology RNA silencing [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics Drosophila melanogaster Gene Expression Regulation Transfer RNA |
| Zdroj: | Nucleic acids research, vol. 48, no. 4, pp. 2050-2072 Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2020, 48 (4), pp.2050-2072. ⟨10.1093/nar/gkaa002⟩ Nucleic Acids Research, Oxford University Press, 2020, ⟨10.1093/nar/gkaa002⟩ |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gkaa002⟩ |
| Popis: | 2′-O-Methylation (Nm) represents one of the most common RNA modifications. Nm affects RNA structure and function with crucial roles in various RNA-mediated processes ranging from RNA silencing, translation, self versus non-self recognition to viral defense mechanisms. Here, we identify two Nm methyltransferases (Nm-MTases) in Drosophila melanogaster (CG7009 and CG5220) as functional orthologs of yeast TRM7 and human FTSJ1. Genetic knockout studies together with MALDI-TOF mass spectrometry and RiboMethSeq mapping revealed that CG7009 is responsible for methylating the wobble position in tRNAPhe, tRNATrp and tRNALeu, while CG5220 methylates position C32 in the same tRNAs and also targets additional tRNAs. CG7009 or CG5220 mutant animals were viable and fertile but exhibited various phenotypes such as lifespan reduction, small RNA pathways dysfunction and increased sensitivity to RNA virus infections. Our results provide the first detailed characterization of two TRM7 family members in Drosophila and uncover a molecular link between enzymes catalyzing Nm at specific tRNAs and small RNA-induced gene silencing pathways. |
| Databáze: | OpenAIRE |
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