Identification of 50 Class D β-Lactamases and 65 Acinetobacter-Derived Cephalosporinases in Acinetobacter spp
| Autor: | Michael Feldgarden, Dominique Clermont, Sylvie Goussard, Cheryl I. Murphy, Violaine Walewski, Bruno Périchon, Gustavo C. Cerqueira, Patrice Courvalin, Lenka Krizova, Alexandr Nemec, Jennifer Wortman |
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| Přispěvatelé: | Agents antibactériens, Institut Pasteur [Paris], National Institute of Public Health [Prague], Broad Institute of MIT and Harvard (BROAD INSTITUTE), Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston], Collection de l'Institut Pasteur (CIP), Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2014 |
| Předmět: |
Amino Acid Motifs
Gene Expression MESH: Recombinant Proteins MESH: Amino Acid Motifs MESH: Cephalosporinase Acinetobacter parvus polycyclic compounds Pharmacology (medical) MESH: Phylogeny Phylogeny Cephalosporinase 0303 health sciences Acinetobacter biology Phylogenetic tree MESH: Escherichia coli Recombinant Proteins Anti-Bacterial Agents Acinetobacter baumannii Infectious Diseases MESH: Acinetobacter Acinetobacter calcoaceticus Acinetobacter lwoffii Plasmids MESH: Gene Expression Molecular Sequence Data MESH: Sequence Alignment beta-Lactams Microbiology 03 medical and health sciences Mechanisms of Resistance MESH: beta-Lactams MESH: Plasmids MESH: Anti-Bacterial Agents Escherichia coli 030304 developmental biology Pharmacology Acinetobacter pittii MESH: Molecular Sequence Data 030306 microbiology [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biochemical phenomena metabolism and nutrition biology.organism_classification rpoB [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics bacteria Sequence Alignment |
| Zdroj: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2014, 58 (2), pp.936-949. ⟨10.1128/AAC.01261-13⟩ Antimicrobial Agents and Chemotherapy, 2014, 58 (2), pp.936-949. ⟨10.1128/AAC.01261-13⟩ |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.01261-13 |
| Popis: | Whole-genome sequencing of a collection of 103 Acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class D β-lactamases and 65 new Acinetobacter -derived cephalosporinases (ADC). All oxacillinases (OXA) contained the three typical motifs of class D β-lactamases, STFK, (F/Y)GN, and K(S/T)G. The phylogenetic tree drawn from the OXA sequences led to an increase in the number of OXA groups from 7 to 18. The topologies of the OXA and RpoB phylogenetic trees were similar, supporting the ancient acquisition of bla OXA genes by Acinetobacter species. The class D β-lactamase genes appeared to be intrinsic to several species, such as Acinetobacter baumannii , Acinetobacter pittii , Acinetobacter calcoaceticus , and Acinetobacter lwoffii . Neither bla OXA-40/143 - nor bla OXA-58 -like genes were detected, and their origin remains therefore unknown. The phylogenetic tree analysis based on the alignment of the sequences deduced from bla ADC revealed five main clusters, one containing ADC belonging to species closely related to A. baumannii and the others composed of cephalosporinases from the remaining species. No indication of bla OXA or bla ADC transfer was observed between distantly related species, except for bla OXA-279 , possibly transferred from Acinetobacter genomic species 6 to Acinetobacter parvus . Analysis of β-lactam susceptibility of seven strains harboring new oxacillinases and cloning of the corresponding genes in Escherichia coli and in a susceptible A. baumannii strain indicated very weak hydrolysis of carbapenems. Overall, this study reveals a large pool of β-lactamases in different Acinetobacter spp., potentially transferable to pathogenic strains of the genus. |
| Databáze: | OpenAIRE |
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