Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro
| Autor: | Yoshiyasu Uchiyama, Kayoko Tono, Akira Akatsuka, Yoshinori Okada, Shuichi Soeda, Maki Masuda, Tetsuro Tamaki, Masahiro Nitta |
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| Rok vydání: | 2011 |
| Předmět: |
Cell type
Histology Muscle Fibers Skeletal Muscle Development MyoD Cell Line Desmin Pathology and Forensic Medicine Mice Laminin Cell Adhesion medicine Animals Myocyte Muscle Skeletal Cells Cultured MyoD Protein biology Reverse Transcriptase Polymerase Chain Reaction Myogenesis Integrin beta1 Gene Expression Regulation Developmental PAX7 Transcription Factor Skeletal muscle Cell Biology musculoskeletal system Molecular biology Mice Inbred C57BL medicine.anatomical_structure Cell culture biology.protein Myogenin Stem cell tissues |
| Zdroj: | Cell and Tissue Research. 344:147-168 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-010-1127-9 |
| Popis: | As a novel approach to distinguish skeletal myogenic cell populations, basal lamina (BL) formation of myogenic cells was examined in the mouse compensatory enlarged plantaris muscles in vivo and in fiber-bundle cultures in vitro. MyoD(+) myogenic cells located inside the regenerative muscle fiber BL were laminin(-) but interstitial MyoD(+) cells were laminin(+). This was also confirmed by electron microscopy as structural BL formation. Similar trends were observed in the fiber-bundle cultures including satellite cells and interstitial myogenic cells and laminin(+) myogenic cells predominantly showed non-adhesive (non-Ad) behavior with Pax7(-), whereas laminin(-) cells were adhesive (Ad) with Pax7(+). Moreover, non-Ad/laminin(+) and Ad/laminin(-) myotubes were also observed and the former type showed spontaneous contractions, while the latter type did not. The origin and hierarchy of Ad/Pax7(+)/laminin(-) and non-Ad/Pax7(-)/laminin(+) myogenic cells were also examined using skeletal muscle interstitium-derived CD34(+)/45(-) (Sk-34) and CD34(-)/45(-) (Sk-DN) multipotent stem cells, which were composed of non-committed myogenic cells with a few (1%) Pax7(+) cells in the Sk-DN cells at fresh isolation. Both cell types were separated by Ad/non-Ad capacity in repetitive culture. As expected, both Ad/Pax7(+)/laminin(-) and non-Ad/Pax7(-)/laminin(+) myogenic cells consistently appeared in the Ad and non-Ad cell culture. However, Ad/Pax7(+)/laminin(-) cells were repeatedly detected in the non-Ad cell culture, while the opposite phenomenon did not occur. This indicates that the source of non-Ad/ Pax7(-)/laminin(+) myogenic cells was present in the Sk-34 and Sk-DN stem cells and they were able to produce Ad/ Pax7(+)/ laminin(-) myogenic cells during myogenesis as primary myoblasts and situated hierarchically upstream of the latter cells. |
| Databáze: | OpenAIRE |
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