Chronic high-fat diet affects intestinal fat absorption and postprandial triglyceride levels in the mouse
| Autor: | Marie-Claude Monnot, Philippe Besnard, Isabelle Niot, Thierry Pineau, Pascal G.P. Martin, Laurent Arnould, Valerie Petit |
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| Přispěvatelé: | Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Département de Biologie et pathologie des tumeurs [Centre Georges-François Leclerc], Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Mitotic index 030309 nutrition & dietetics Linoleic acid QD415-436 Biology Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound Endocrinology SMALL INTESTINE INTESTINAL PROLIFERATION LIPID BINDING PROTEINS TRIGLYCERIDEMIA Triglyceridemia Internal medicine medicine Animals Tyloxapol Triglycerides Cell Proliferation 030304 developmental biology 2. Zero hunger chemistry.chemical_classification 0303 health sciences Triglyceride Fatty Acids Lipid binding proteins Fatty acid Lipid metabolism Small intestine Cell Biology Lipid Metabolism Postprandial Period Dietary Fats Steatorrhea Diet Jejunum Postprandial medicine.anatomical_structure Gene Expression Regulation Intestinal Absorption chemistry Intestinal proliferation lipids (amino acids peptides and proteins) [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition medicine.drug |
| Zdroj: | Journal of Lipid Research 2 (48), 278-287. (2007) Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 2007, 48 (2), pp.278-287. ⟨10.1194/jlr.M600283-JLR200⟩ Journal of Lipid Research, Vol 48, Iss 2, Pp 278-287 (2007) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.M600283-JLR200⟩ |
| Popis: | International audience; The effects of chronic fat overconsumption on intestinal physiology and lipid metabolism remain elusive. It is unknown whether a fat-mediated adaptation to lipid absorption takes place. To address this issue, mice fed a high-fat diet (40%, w/w) were refed or not a control diet (3%, w/w) for 3 additive weeks. Despite daily lipid intake 7.7-fold higher than in controls, fecal lipid output remained unchanged in mice fed the triglyceride (TG)-rich diet. In situ isolated jejunal loops revealed greater [1-(14)C]linoleic acid uptake without TG accumulation in mucosa, suggesting an increase in lipid absorption capacity. Induction both in intestinal mitotic index and in the expression of genes involved in fatty acid uptake, trafficking, and lipoprotein synthesis was found in high-fat diet mice. These changes were lipid-mediated, in that they were fully abolished in mice refed the control diet. A lipid load test performed in the presence or absence of the LPL inhibitor tyloxapol showed a sustained blood TG clearance in fat-fed mice likely attributable to intestinal modulation of LPL regulators (apolipoproteins C-II and C-III). These data demonstrate that a chronic high-fat diet greatly affects intestinal physiology and body lipid use in the mouse. |
| Databáze: | OpenAIRE |
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