High prevalence of human cytomegalovirus proteins and nucleic acids in primary breast cancer and metastatic sentinel lymph nodes

Autor: Cecilia Söderberg-Nauclér, Jan Frisell, Jana de Boniface, Koon-Chu Yaiw, Chato Taher, Afsar Rahbar, Abdul-Aleem Mohammad, Johan Hartman, Piotr Religa
Rok vydání: 2012
Předmět:
Human cytomegalovirus
Pathology
viruses
lcsh:Medicine
Cytomegalovirus
Pathogenesis
medicine.disease_cause
Metastasis
Emerging Viral Diseases
lcsh:Science
Multidisciplinary
Cancer Risk Factors
virus diseases
Obstetrics and Gynecology
Middle Aged
Immunohistochemistry
Host-Pathogen Interaction
Oncology
Medical Microbiology
Medicine
Female
Lymph
Research Article
Adult
medicine.medical_specialty
Sentinel lymph node
Breast Neoplasms
In Vitro Techniques
Real-Time Polymerase Chain Reaction
Microbiology
Viral Proteins
Breast cancer
Virology
Progesterone receptor
Breast Cancer
medicine
Humans
Biology
Microbial Pathogens
Aged
business.industry
Sentinel Lymph Node Biopsy
lcsh:R
biochemical phenomena
metabolism
and nutrition
medicine.disease
Viruses and Cancer
Microbial Evolution
lcsh:Q
Lymph Nodes
Carcinogenesis
business
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 2, p e56795 (2013)
ISSN: 1932-6203
Popis: Background Breast cancer is a leading cause of death among women worldwide. Increasing evidence implies that human cytomegalovirus (HCMV) infection is associated with several malignancies. We aimed to examine whether HCMV is present in breast cancer and sentinel lymph node (SLN) metastases. Materials and Methods Formalin-fixed paraffin-embedded tissue specimens from breast cancer and paired sentinel lymph node (SLN) samples were obtained from patients with (n = 35) and without SLN metastasis (n = 38). HCMV immediate early (IE) and late (LA) proteins were detected using a sensitive immunohistochemistry (IHC) technique and HCMV DNA by real-time PCR. Results HCMV IE and LA proteins were abundantly expressed in 100% of breast cancer specimens. In SLN specimens, 94% of samples with metastases (n = 34) were positive for HCMV IE and LA proteins, mostly confined to neoplastic cells while some inflammatory cells were HCMV positive in 60% of lymph nodes without metastases (n = 35). The presence of HCMV DNA was confirmed in 12/12 (100%) of breast cancer and 10/11 (91%) SLN specimens from the metastatic group, but was not detected in 5/5 HCMV-negative, SLN-negative specimens. There was no statistically significant association between HCMV infection grades and progesterone receptor, estrogen receptor alpha and Elston grade status. Conclusions The role of HCMV in the pathogenesis of breast cancer is unclear. As HCMV proteins were mainly confined to neoplastic cells in primary breast cancer and SLN samples, our observations raise the question whether HCMV contributes to the tumorigenesis of breast cancer and its metastases.
Databáze: OpenAIRE
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