High prevalence of human cytomegalovirus proteins and nucleic acids in primary breast cancer and metastatic sentinel lymph nodes
Autor: | Cecilia Söderberg-Nauclér, Jan Frisell, Jana de Boniface, Koon-Chu Yaiw, Chato Taher, Afsar Rahbar, Abdul-Aleem Mohammad, Johan Hartman, Piotr Religa |
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Rok vydání: | 2012 |
Předmět: |
Human cytomegalovirus
Pathology viruses lcsh:Medicine Cytomegalovirus Pathogenesis medicine.disease_cause Metastasis Emerging Viral Diseases lcsh:Science Multidisciplinary Cancer Risk Factors virus diseases Obstetrics and Gynecology Middle Aged Immunohistochemistry Host-Pathogen Interaction Oncology Medical Microbiology Medicine Female Lymph Research Article Adult medicine.medical_specialty Sentinel lymph node Breast Neoplasms In Vitro Techniques Real-Time Polymerase Chain Reaction Microbiology Viral Proteins Breast cancer Virology Progesterone receptor Breast Cancer medicine Humans Biology Microbial Pathogens Aged business.industry Sentinel Lymph Node Biopsy lcsh:R biochemical phenomena metabolism and nutrition medicine.disease Viruses and Cancer Microbial Evolution lcsh:Q Lymph Nodes Carcinogenesis business |
Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 2, p e56795 (2013) |
ISSN: | 1932-6203 |
Popis: | Background Breast cancer is a leading cause of death among women worldwide. Increasing evidence implies that human cytomegalovirus (HCMV) infection is associated with several malignancies. We aimed to examine whether HCMV is present in breast cancer and sentinel lymph node (SLN) metastases. Materials and Methods Formalin-fixed paraffin-embedded tissue specimens from breast cancer and paired sentinel lymph node (SLN) samples were obtained from patients with (n = 35) and without SLN metastasis (n = 38). HCMV immediate early (IE) and late (LA) proteins were detected using a sensitive immunohistochemistry (IHC) technique and HCMV DNA by real-time PCR. Results HCMV IE and LA proteins were abundantly expressed in 100% of breast cancer specimens. In SLN specimens, 94% of samples with metastases (n = 34) were positive for HCMV IE and LA proteins, mostly confined to neoplastic cells while some inflammatory cells were HCMV positive in 60% of lymph nodes without metastases (n = 35). The presence of HCMV DNA was confirmed in 12/12 (100%) of breast cancer and 10/11 (91%) SLN specimens from the metastatic group, but was not detected in 5/5 HCMV-negative, SLN-negative specimens. There was no statistically significant association between HCMV infection grades and progesterone receptor, estrogen receptor alpha and Elston grade status. Conclusions The role of HCMV in the pathogenesis of breast cancer is unclear. As HCMV proteins were mainly confined to neoplastic cells in primary breast cancer and SLN samples, our observations raise the question whether HCMV contributes to the tumorigenesis of breast cancer and its metastases. |
Databáze: | OpenAIRE |
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