RNA-seq analysis reveals that oleic acid exerts anti-inflammatory effects via modulating cell cycle in Raw264.7 cells
| Autor: | Xiao-Jun Li, Wei Kevin Zhang, Taoyu Li, Wan-Li Liang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
lcsh:R5-920
medicine.diagnostic_test Lipopolysaccharide Cell cycle control medicine.drug_class Cell cycle process p21Cip1/Waf1 Cell cycle Oleic acid Anti-inflammatory Cell biology Transcriptome chemistry.chemical_compound Western blot chemistry lcsh:Biology (General) Apoptosis medicine Secretion lipids (amino acids peptides and proteins) lcsh:Medicine (General) lcsh:QH301-705.5 |
| Zdroj: | STEMedicine, Vol 2, Iss 6 (2021) |
| ISSN: | 2705-1188 |
| Popis: | Background: Lipopolysaccharide (LPS), a structural and protective compound primarily found in ordinary benign bacteria, could induce pro-inflammatory effect in a macrophage cell line Raw264.7 cells. Additionally, we previously showed that oleic acid (OA) possessed apoptotic effect in mouseoriginated Raw264.7 cells as well. Methods: Cellular and molecular methods including flowcytometry and Western blot as well as bioinformatic methods including RNAseq and analysis. Results: We demonstrated that OA could alleviate LPS-activated inflammatory effects, including apoptosis and secretion of cytokines via the modulation of cell cycle process. Further analysis revealed that OA reduced the LPS-elevated expression of p21, but not p16. Conclusion: Our investigation has provided detailed information on LPS stimulation and OA remission in Raw264.7 cells, and laid solid foundation for the potential pharmaceutical application of OA as an antiinflammatory agent. |
| Databáze: | OpenAIRE |
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