Tumor-stroma interaction increases CD147 expression in neoplastic B lymphocytes in chronic lymphocytic leukemia
| Autor: | Cecilia M. Rodriguez, Ana Carolina Donadio, Mónica Beatriz Gilardoni, Viviana Heller, Darío Sastre, Claudia Gabriela Pellizas, María Mónica Remedi |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Stromal cell Chronic lymphocytic leukemia Cell Cell Communication Matrix metalloproteinase MMP9 Flow cytometry immune system diseases hemic and lymphatic diseases medicine Humans Fibroblast neoplasms Molecular Biology B-Lymphocytes medicine.diagnostic_test Gene Expression Regulation Leukemic Chemistry Cell migration Cell Biology Hematology medicine.disease Leukemia Lymphocytic Chronic B-Cell Neoplasm Proteins medicine.anatomical_structure Basigin Cancer research Molecular Medicine Female Stromal Cells |
| Zdroj: | Blood Cells, Molecules, and Diseases. 82:102405 |
| ISSN: | 1079-9796 |
| Popis: | Background Chronic lymphocytic leukemia (CLL) microenvironment plays a critical role in disease pathogenesis. Matrix metalloproteinases (MMPs) are involved in CLL-B cell migration and survival. CD147 is associated with MMPs production by tumor and stromal cells. Aim To analyze CD147, MMP2 and MMP9 expression in CLL-B cells and its modulation by fibroblasts (Fb)-CLL-B cell interaction. Methods CLL-B cells were co-cultured with Fb, as a simulation of CLL microenvironment. CD147 was evaluated in healthy donor (HD)-B cells and CLL-B cells by flow cytometry. MMP2 and MMP9 activity in CLL-plasma samples and conditioned media (CMs) was studied by zymography. Results MMP9/MMP2 plasma levels were significantly higher in CLL patients than in HD. CD147 expression (median fluorescence intensity) in CLL patients characterized 3 groups: low- (19.1 ± 3.2; n=3), middle- (42.7 ± 12.8; n=18) and high- (76.5 ± 9.6; n=5) related to CD147 expression in HD-B cells. CD147 expression significantly increased in CLL-B cells after Fb-CLL-B cell co-culture. A significant increase in proMMP2 activity was observed in CMs obtained from Fb-CLL-B cell co-cultures in comparison with isolated CLL-B cells. Conclusions CD147 expression in CLL-B cells and MMPs secretion was induced by Fb-CLL-B cell contact, suggesting CD147 participation in the CLL pathophysiology. |
| Databáze: | OpenAIRE |
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