Cytotoxicity, Anti-Obesity and Anti-Diabetic Activities of Heteromorpha arborescens (Spreng.) Cham Leaves
| Autor: | Anthony Jide Afolayan, Taiwo Oluwafunmilola Abifarin, Gloria Aderonke Otunola |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Glucose utilization
Bioengineering TP1-1185 chemistry.chemical_compound parasitic diseases lipase Chemical Engineering (miscellaneous) Food science Heteromorpha arborescens Lipase Cytotoxicity QD1-999 IC50 Aqueous solution Ethanol biology Chemistry Chemical technology Process Chemistry and Technology body regions glucose utilization α-amylase diabetes mellitus Toxicity biology.protein cytotoxicity α-glucosidase |
| Zdroj: | Processes, Vol 9, Iss 1671, p 1671 (2021) Processes Volume 9 Issue 9 |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr9091671 |
| Popis: | This study investigated the cytotoxicity, anti-obesity and anti-diabetic potentials of blanched, aqueous and ethanol extracts of Heteromorpha arborescens (Spreng.) Cham leaves. The results revealed that both ethanol and aqueous extracts exhibited considerable inhibition against α-glucosidase (IC50 of 627.29 ± 4.62 µg/mL and 576.46 ± 3.21 µg/mL respectively), while the blanched extract showed weak α-glucosidase inhibition (IC50 855.38 ± 4.29 µg/mL) and the aqueous extract showed the best α-amylase inhibition (IC50 583.74 ± 5.87 µg/mL). However, weak α-amylase inhibition was observed in the ethanol (IC50 724.60 ± 4.33 µg/mL) and blanched extracts (IC50 791.63 ± 3.76 µg/mL). The toxicity of the extracts is indicated by LC50 values as 154.75 µg/mL, 125 µg/mL and 90.58 µg/mL for ethanol, aqueous and blanched extracts respectively, indicating the blanched extract to be the most toxic. Moderate glucose utilization in both C3A and L6 cells was also observed for the aqueous and ethanol extracts which may be attributed to the relatively lower toxicity levels present. However, glucose utilization was very weak for the blanched extract, which may be due to higher level of cytotoxicity it possessed. Relatively weaker lipase inhibition was observed for the ethanol (IC50 699.3 ± 1.33 µg/mL), aqueous (IC50 811.52 ± 3.52 µg/mL) and blanched extracts (IC50 1152.7 ± 4.61 µg/mL) compared to orlistat (IC50 56.88 ± 0.11 µg/mL). However, there was no reasonable reduction in lipid accumulation observed in all the extract treated cells. These observations suggest that ethanol and aqueous extracts of H. arborescens leaf are promising as new agents for the treatment of diabetes and its acclaimed anti-obesity potentials are likely due to its lipase, α-amylase and α-glucosidase inhibition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|