| Autor: |
Lin Ji, Xisha Lin, Kenan Yuan, Yue Li, Ambreen Leghari, Bingbing Yuan, Huixing Lin |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Microbial Pathogenesis. 172:105801 |
| ISSN: |
0882-4010 |
| DOI: |
10.1016/j.micpath.2022.105801 |
| Popis: |
Salmonella spp. poses a great threat to the livestock, food safety and public health. A recombinant swinepox virus expressing a protective antigen sseB was constructed by homologous recombination to develop a vaccine against Salmonella infection. The rSPV-sseB was verified using PCR, Western blot and indirect immunofluorescence assay. The immune responses and protective efficacy of rSPV-sseB were assessed in piglets. Forty piglets were immunized with rSPV-sseB, inactive Salmonella vaccine, wild-type SPV (wtSPV), or PBS. The results showed that the level of the sseB-specific antibody of the rSPV-sseB-vaccinated piglets was significantly higher at all time points post-vaccination than those of the inactivated Salmonella vaccine (P 0.05), wtSPV (P 0.001) or mock treated piglets (P 0.001). The IL-4 and IFN-γ in the rSPV-sseB group were significantly higher than the other three groups at all post-infection time points. rSPV-sseB provided piglets with strong protection against the challenge of S. typhimurium with lethal dose. These results suggest the possibility of using recombinant swinepox virus rSPV-sseB as a promising vaccine to prevent Salmonella infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect