Adenosine 3',5'-cyclic monophosphate dependent and independent protein kinase activities in 1,2-dimethylhydrazine induced rat colon cancer
| Autor: | R. H. Stevens, D. P. Loven, D. Singh |
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| Rok vydání: | 1982 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Phosvitin Toxicology chemistry.chemical_compound Internal medicine medicine Cyclic AMP Animals Protein kinase A chemistry.chemical_classification Dimethylhydrazines Cell growth Kinase General Medicine Neoplasms Experimental Hydrogen-Ion Concentration Adenosine 1 2-Dimethylhydrazine Neoplasm Proteins Rats Kinetics Endocrinology Enzyme chemistry Colonic Neoplasms Protein Kinases Intracellular medicine.drug |
| Zdroj: | Chemico-biological interactions. 40(2) |
| ISSN: | 0009-2797 |
| Popis: | The adenosine 3',5'-cyclic monophosphate (cAMP)-dependent and cAMP-independent kinase activities were measured in the 1,2-dimethylhydrazine (DMH) induced rat colon cancer and in untreated colon. Previous studies had shown that intestinal tumors induced by chronic exposure to DMH contained 2-fold less intracellular cAMP. The present findings indicate that reduction in cAMP-dependent protein kinase activities also occur in colon cancer cells. Similar hydrogen ion dependence (pH 6-7) and approximate association constants (Ka approximately 0.1 microM) were observed for the enzymes existing in both normal and tumor tissues, while the cAMP-dependent tumor protein kinase was found to phosphorylate phosvitin and casein to a greater degree. These recent findings are consistent with the concept that the concentrations of cAMP and activities of its associated enzyme system are inversely related to the cell proliferation state. |
| Databáze: | OpenAIRE |
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