Efficient methodology for the cyclization of linear peptide libraries via intramolecularS-alkylation using Multipin™ solid phase peptide synthesis
| Autor: | Nicholas J. Ede, John N. Lambert, Andrew M. Bray, Kade D. Roberts |
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| Rok vydání: | 2006 |
| Předmět: |
Alkylation
Stereochemistry EcoRI Peptide Peptides Cyclic Biochemistry Mass Spectrometry chemistry.chemical_compound Peptide Library Structural Biology Drug Discovery Peptide synthesis Combinatorial Chemistry Techniques Amino Acid Sequence Peptide library Molecular Biology Peptide sequence Pharmacology chemistry.chemical_classification Molecular Structure biology Organic Chemistry Reproducibility of Results General Medicine Combinatorial chemistry Cyclic peptide EcoRV chemistry Cyclization Intramolecular force biology.protein Molecular Medicine Chromatography Liquid |
| Zdroj: | Journal of Peptide Science. 12:525-532 |
| ISSN: | 1099-1387 1075-2617 |
| DOI: | 10.1002/psc.761 |
| Popis: | Methodology is described here for the efficient parallel synthesis and cyclization of linear peptide libraries using intramolecular S-alkylation chemistry in combination with Multipin solid phase peptide synthesis (Multipin SPPS). The effective use of this methodology was demonstrated with the synthesis of a 72-member combinatorial library of cyclic thioether peptide derivatives of the conserved four-residue structural motif DD/EXK found in the active sites of the five crystallographically defined orthodox type II restriction endonucleases, EcoRV, EcoRI, PvuII, BamHI and BglI. |
| Databáze: | OpenAIRE |
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