Staphylococcus aureus requires less virulence to establish an infection in diabetic hosts
| Autor: | Eva Korpos, Lydia Sorokin, Georg Peters, Anke Siegmund, Stefanie Deinhardt-Emmer, Olaf Bach, Clais R. Findeisen, Hélène Van de Vyver, Salome Kherkheulidze, Bettina Löffler, Martin Rindert, Cord Sunderkötter, Lorena Tuchscherr, Alexander Mellmann |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Microbiology (medical) Staphylococcus aureus Virulence Biology medicine.disease_cause Microbiology Streptozocin Diabetes Mellitus Experimental Mice 03 medical and health sciences Immune system Mice Inbred NOD Diabetes mellitus Human Umbilical Vein Endothelial Cells medicine Animals Humans Pathogen Cells Cultured Aged NOD mice Soft Tissue Infections General Medicine Middle Aged medicine.disease Bacterial Load Diabetic Foot In vitro Mice Inbred C57BL 030104 developmental biology Infectious Diseases Cell culture Hyperglycemia |
| Zdroj: | International Journal of Medical Microbiology. 308:761-769 |
| ISSN: | 1438-4221 |
| DOI: | 10.1016/j.ijmm.2018.05.004 |
| Popis: | Staphylococcus aureus is the most frequent pathogen causing diabetic foot infections. Here, we investigated the degree of bacterial virulence required to establish invasive tissue infections in diabetic organisms. Staphylococcal isolates from diabetic and non-diabetic foot ulcers were tested for their virulence in in vitro functional assays of host cell invasion and cytotoxicity. Isolates from diabetes mellitus type I/II patients exhibited less virulence than isolates from non-diabetic patients, but were nevertheless able to establish severe infections. In some cases, non-invasive isolates were detected deep within diabetic wounds, even though the strains were non-pathogenic in cell culture models. Testing of defined isolates in murine footpad injection models revealed that both low- and high-virulent bacterial strains persisted in higher numbers in diabetic compared to non-diabetic hosts, suggesting that hyperglycemia favors bacterial survival. Additionally, the bacterial load was higher in NOD mice, which have a compromised immune system, compared to C57Bl/6 mice. Our results reveal that high as well as low-virulent staphylococcal strains are able to cause soft tissue infections and to persist in diabetic humans and mice, suggesting a reason for the frequent and endangering infections in patients with diabetes. |
| Databáze: | OpenAIRE |
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