Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng
| Autor: | You-Kun Zheng, Ze-Yan Fan, Hua-Hong Chen, Xin-Guo Qiao, Li-Hua Xu, Hui-Lin Guan, Li-Xing Zhao, You-Wei Chen, Cui-Ping Miao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Panax notoginseng Rhizobacteria 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) complex mixtures diversity 03 medical and health sciences lcsh:Botany Root rot Plant defense against herbivory rhizobacteria biology business.industry food and beverages biology.organism_classification Biotechnology lcsh:QK1-989 030104 developmental biology Complementary and alternative medicine antagonistic activities business 010606 plant biology & botany Research Article |
| Zdroj: | Journal of Ginseng Research, Vol 40, Iss 2, Pp 97-104 (2016) Journal of Ginseng Research |
| ISSN: | 1226-8453 |
| Popis: | Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum. |
| Databáze: | OpenAIRE |
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