Serological investigation of racehorse vaccination against equine influenza in Morocco
Autor: | Romain Paillot, Wissal Mahir, Mohamed Dilai, Loïc Legrand, Mehdi El Harrak, Stéphanie Fougerolle, Ouafaa Fassi Fihri, Mohammed Piro, Rachida El Mourid |
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Přispěvatelé: | Department of Medicine, Surgery and Reproduction, Institut Agronomique et Vétérinaire Hassan II - IAV (MOROCCO) (IAV), Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), M.C.I Animal Health, Department of Microbiology, Immunology and Contagious Diseases |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Veterinary medicine 040301 veterinary sciences SRH education Equine influenza Enzyme-Linked Immunosorbent Assay Equine influenza virus Biology Antibodies Viral Microbiology Serology HI 0403 veterinary science 03 medical and health sciences Vaccination status Racehorses Orthomyxoviridae Infections Morocco Single radial haemolysis Animals Horses Hemagglutination assay General Veterinary [SDV.BA]Life Sciences [q-bio]/Animal biology Vaccination 04 agricultural and veterinary sciences General Medicine Hemagglutination Inhibition Tests Mandatory vaccination 3. Good health 030104 developmental biology Influenza A virus Influenza Vaccines ELISA Horse Diseases Protective antibody |
Zdroj: | Veterinary Microbiology Veterinary Microbiology, Elsevier, 2018, 223, pp.153-159. ⟨10.1016/j.vetmic.2018.08.014⟩ |
ISSN: | 1873-2542 0378-1135 |
Popis: | International audience; In order to evaluate the vaccination status against equine influenza (EI) in Moroccan racehorses, a serological investigation was carried out on 509 racehorses using three serological tests: an Enzyme-Linked Immunosorbent Assay (ELISA), the Hemagglutination Inhibition (HI) test and the Single Radial Haemolysis (SRH) assay. The serological analysis showed 56% of seropositivity by ELISA, 67% by HI and 89.4% by SRH (with 69.9% above the clinical protection threshold). Using the Kappa test, the SRH and HI assays showed a strong agreement, the SRH and ELISA assays had a moderate agreement and the HI and ELISA assays showed a poor agreement. Seropositivity was positively correlated with the age of horses and the number of immunisation received. EI vaccines used during the last immunisation before the study had a weak influence on the serological status. This effect was observed when the vaccines Calvenza and Fluvac Innovator® were used, with 94.1% and 100% of seropositivity when measured by HI, and with 100% and 94.7% exceeding the clinical protection threshold when measured by SRH, respectively. No effect was found when other EI vaccines, including Prequenza-Te® (67% coverage (342/509) and Proteqflu-Te® (22% coverage (114/509) were used; with 64% and 67.5% seropositivity (HI) and with 66.4% and 72.8% above the clinical threshold (SRH), respectively. The location and the time since last vaccination have no influence on the serological result. Overall, levels of protective antibody against EI in Moroccan racehorses remain a concern despite mandatory vaccination. |
Databáze: | OpenAIRE |
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