Molecular cloning and characterization of a Spirometra erinacei casein kinase I
| Autor: | Man Wang, Peng Jiang, Li Na Liu, Li Fu Wang, Xin Qi, Xi Zhang, Ming Guo Xu, Zhong Quan Wang, Jing Cui, Han Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Blotting
Western Molecular cloning law.invention Mice Antigen law Casein Kinase I Plerocercoid Escherichia coli Animals Humans Cloning Molecular Spirometra Mice Inbred BALB C General Veterinary biology Helminth Proteins General Medicine Viral tegument Cestode Infections biology.organism_classification Molecular biology Recombinant Proteins Blot Infectious Diseases Insect Science Recombinant DNA Female Immunization Parasitology |
| Zdroj: | Parasitology Research. 114:4737-4742 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-015-4758-7 |
| Popis: | The Spirometra erinacei casein kinase I (SeCKI) gene was cloned and expressed in Escherichia coli, and its characteristics were investigated in this study. The recombinant SeCP protein (rSeCKI) was purified. The vaccination of mice with rSeCKI induced the Th1/Th2-mixed type of immune response with Th2 predominant (high levels of IgG1). Western blotting analysis showed that rSeCP was recognized by the sera of plerocercoid-infected mice, and anti-rSeCP serum recognized the native SeCP protein of plerocercoid crude antigens. Transcription and expression of SeCP was observed at the plerocercoid and adult stages of S. erinacei. Immunolocalization identified SeCKI in the tegument and parenchymal tissues of plerocercoids and in the teguments of adults. SeCKI appeared to be essential indispensable for the S. erinacei development and survival in host, but its biological functions need to be further investigated. |
| Databáze: | OpenAIRE |
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