Immunization of A4galt-deficient mice with glycosphingolipids from renal cell cancers resulted in the generation of anti-sulfoglycolipid monoclonal antibodies
| Autor: | Noboru Hashimoto, Kazutaka Ikeda, Koichi Furukawa, Akemi Suzuki, Reiko Ando, Momokazu Goto, Tokunori Yamamoto, Xiaoen Fan, Ryo Taguchi, Yukio Niimura, Noriyo Tokuda, Keiko Furukawa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antibodies Neoplasm medicine.drug_class Cell Mice Nude urologic and male genital diseases Monoclonal antibody Biochemistry Antibodies Monoclonal Murine-Derived Mice 03 medical and health sciences Glycolipid Antigen Cell Line Tumor medicine Animals Humans Molecular Biology Mice Knockout Mice Inbred BALB C Kidney Chemistry Cell Biology Galactosyltransferases Molecular biology Kidney Neoplasms female genital diseases and pregnancy complications 030104 developmental biology medicine.anatomical_structure Immunization Cell culture Immunohistochemistry lipids (amino acids peptides and proteins) Glycolipids |
| Zdroj: | Glycoconjugate Journal. 33:169-180 |
| ISSN: | 1573-4986 0282-0080 |
| DOI: | 10.1007/s10719-016-9654-6 |
| Popis: | In this study, we immunized Gb3/CD77 synthase gene (A4galt) knockout (KO) mice with glycosphingolipids (GSLs) extracted from 3 renal cell cancer (RCC) cell lines to raise monoclonal antibodies (mAbs) reactive with globo-series GSLs specifically expressed in RCCs. Although a number of mAbs reactive with globo-series GSLs were generated, they reacted with both RCC cell lines and normal kidney cells. When we analyzed recognized antigens by mAbs that were specifically reactive with RCC, but not with normal kidney cells at least on the cell surface, many of them turned out to be reactive with sulfoglycolipids. Eight out of 11 RCC-specific mAbs were reactive with SM2 alone, and the other 3 mAbs were more broadly reactive with sulfated glycolipids, i.e. SM3 and SM4 as well as SM2. In the immunohistochemistry, these anti-sulfoglycolipids mAbs showed RCC-specific reaction, with no or minimal reaction with adjacent normal tissues. Thus, immunization of A4galt KO mice with RCC-derived GSLs resulted in the generation of anti sulfated GSL mAbs, and these mAbs may be applicable for the therapeutics for RCC patients. |
| Databáze: | OpenAIRE |
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