Isolation of naphthazarin from walnut 'onigurumi' and its inhibitory action on oxidative phosphorylation in mitochondria
| Autor: | Yuji Inada, Nobuo Ikekawa, Akihiro Ohta, Sein Lin, P.M. Sivalingam, Nakatsugu Yaginuma |
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| Rok vydání: | 1973 |
| Předmět: |
Magnetic Resonance Spectroscopy
Spectrophotometry Infrared Oryzias Mitochondria Liver Oxidative phosphorylation Naphthols Mitochondrion In Vitro Techniques Toxicology Oxidative Phosphorylation chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Animals Killifish Toxins Biological Chromatography biology Dose-Response Relationship Drug Fishes Phosphorus Isotopes Fish toxins Plants biology.organism_classification Rats Juglans mandshurica chemistry Biochemistry Toxicity Calcium Juglone Hydrogen Naphthoquinones |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 11(3) |
| ISSN: | 0041-0101 |
| Popis: | Investigation of lethal substances other than juglone in the walnut ‘Onigurumi’, Juglans mandshurica maxim var. Sieboldiana Makino, led to the isolation and identification for the first time, of 5,8-dihydroxy-1,4-naphthoquinone (naphthazarin) in plants. Toxicity experiments with both these fish toxins on albino orange-red killifish ‘Himedaka’, Oryzias latipes , gave median tolerance limits (TL m ) of 0.245 and 0.435 ppm, respectively. A study on the inhibitory effect of these toxins on oxidative phosphorylation showed half maximal inhibitin values ranging between 10 -4 and 10 -5 M for naphthazarin and between 10 -5 and 10 -6 M for juglone. Naphthazarin had complicated dualistic effect acting both as an uncoupler and energy transfer inhibitor, while juglone was only an energy transfer inhibitor. |
| Databáze: | OpenAIRE |
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