Population genetic analysis and trichothecene profiling of Fusarium graminearum from wheat in Uruguay
| Autor: | A Mionetto, D. Pan, L Bettucci, M M Reynoso, N Calero, A Torres |
|---|---|
| Přispěvatelé: | Pan Dinorah, Universidad de la República (Uruguay). Facultad de Ciencias, Mionetto Cabrera Ana Claudia, Universidad de la República (Uruguay). Facultad de Ciencias, Calero Natalia, Universidad de la República (Uruguay). Facultad de Ciencias, Reynoso M.M., Torres A., Bettucci Rossi Lina Julia, Universidad de la República (Uruguay). Facultad de Ciencias |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Genotype Trichothecene Population 01 natural sciences 03 medical and health sciences Gene Expression Regulation Fungal Genetic variation Genetics Genetic variability education DNA Fungal Molecular Biology Genotyping Phylogeny Triticum education.field_of_study biology business.industry Gene Expression Profiling Fungal genetics food and beverages Genetic Variation General Medicine Mycotoxins biology.organism_classification Biotechnology Fusarium graminearum Fusarium head blight 030104 developmental biology Genetics Population Wheat Uruguay Genetic structure business Trichothecenes 010606 plant biology & botany |
| Zdroj: | COLIBRI Universidad de la República instacron:Universidad de la República |
| ISSN: | 1676-5680 |
| Popis: | Fusarium graminearum sensu stricto (F. graminearum s.s.) is the major causal agent of Fusarium head blight of wheat worldwide, and contaminates grains with trichothecene mycotoxins that cause serious threats to food safety and animal health. An important aspect of managing this pathogen and reducing mycotoxin contamination of wheat is knowledge regarding its population genetics. Therefore, isolates of F. graminearum s.s. from the major wheat-growing region of Uruguay were analyzed by amplified fragment length polymorphism assays, PCR genotyping, and chemical analysis of trichothecene production. Of the 102 isolates identified as having the 15-ADON genotype via PCR genotyping, all were DON producers, but only 41 strains were also 15-ADON producers, as determined by chemical analysis. The populations were genotypically diverse but genetically similar, with significant genetic exchange occurring between them. Analysis of molecular variance indicated that most of the genetic variability resulted from differences between isolates within populations. Multilocus linkage disequilibrium analysis suggested that the isolates had a panmictic population genetic structure and that there is significant recombination occurs in F. graminearum s.s. In conclusion, tour findings provide the first detailed description of the genetic structure and trichothecene production of populations of F. graminearum s.s. from Uruguay, and expands our understanding of the agroecology of F. graminearum and of the correlation between genotypes and trichothecene chemotypes. |
| Databáze: | OpenAIRE |
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