Prevalence and characterisation of Staphylococcus aureus causing community-acquired skin and soft tissue infections on Java and Bali, Indonesia

Autor: Harun Al Rasyid, Juliëtte A. Severin, Ketut Suata, Nanik Setijowati, Cita Rosita Sigit Prakoeswa, Damayanti Damayanti, Priyo Budi Purwono, Dicky B. Widhyatmoko, Josephine W. I. van Nierop, Susan V. Snijders, Geraldine L. Nanninga, Mitchell Laurens, Michelle de Regt, Nyoman S. Budayanti, Sanarto Santoso, Kuntaman Kuntaman, Neline Oudenes, Dewi Santosaningsih, Henri A. Verbrugh
Přispěvatelé: Medical Microbiology & Infectious Diseases
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
Male
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
030231 tropical medicine
030106 microbiology
Biology
Skin infection
medicine.disease_cause
Staphylococcal infections
Microbiology
03 medical and health sciences
Young Adult
0302 clinical medicine
All institutes and research themes of the Radboud University Medical Center
Bacterial Proteins
medicine
Prevalence
Humans
Penicillin-Binding Proteins
SCCmec
Soft Tissue Infections
Public Health
Environmental and Occupational Health
respiratory system
biochemical phenomena
metabolism
and nutrition
Staphylococcal Infections
bacterial infections and mycoses
medicine.disease
Virology
Methicillin-resistant Staphylococcus aureus
Anterior nares
Community-Acquired Infections
Infectious Diseases
medicine.anatomical_structure
Indonesia
bacteria
Multilocus sequence typing
Parasitology
Panton–Valentine leukocidin
Rare cancers Radboud Institute for Health Sciences [Radboudumc 9]
Zdroj: Tropical Medicine & International Health, 23, 1, pp. 34-44
Tropical Medicine and International Health, 23(1), 34-44. Wiley-Blackwell Publishing Ltd
Tropical Medicine & International Health, 23, 34-44
ISSN: 1360-2276
Popis: textabstractObjectives: To define the role of Staphylococcus aureus in community settings among patients with skin and soft tissue infections (SSTI) in Indonesia. Methods: Staphylococcus aureus were cultured from anterior nares, throat and wounds of 567 ambulatory patients presenting with SSTI. The mecA gene and genes encoding Panton-Valentine leukocidin (PVL; lukF-PV and lukS-PV) and exfoliative toxin (ET; eta and etb) were determined by PCR. Clonal relatedness among methicillin-resistant S. aureus (MRSA) and PVL-positive S. aureus was analysed using multilocus variable-number tandem-repeat analysis (MLVA) typing, and multilocus sequence typing (MLST) for a subset of isolates. Staphylococcal cassette chromosome mec (SCCmec) was determined for all MRSA isolates. Moreover, determinants for S. aureus SSTI, and PVL/ET-positive vs PVL/ET-negative S. aureus were assessed. Results: Staphylococcus aureus were isolated from SSTI wounds of 257 (45.3%) patients, eight (3.1%) of these were MRSA. Genes encoding PVL and ETs were detected in 21.8% and 17.5% of methicillin-susceptible S. aureus (MSSA), respectively. PVL-positive MRSA was not detected. Nasopharyngeal S. aureus carriage was an independent determinant for S. aureus SSTI (odds ratio [OR] 1.8). Primary skin infection (OR 5.4) and previous antibiotic therapy (OR 3.5) were associated with PVL-positive MSSA. Primary skin infection (OR 2.2) was the only factor associated with ET-positive MSSA. MLVA typing revealed two more prevalent MSSA clusters. One ST1-MRSA-SCCmec type IV isolate and a cluster of ST239-MRSA-SCCmec type III were found. Conclusions: Community-acquired SSTI in Indonesia was frequently caused by PVL-positive MSSA, and the hospital-associated ST239-MRSA may have spread from the hospital into the community.
Databáze: OpenAIRE
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