Lipidomic analysis of human placental Syncytiotrophoblast microvesicles in adverse pregnancy outcomes
| Autor: | Anita Sugam Kale, Sonia Baig, Markus R. Wenk, S. Vasoo, Lin Lin Su, Arijit Biswas, Guanghou Shui, J.Y. Lim, Aaron Z. Fernandis, Mahesh Choolani |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Abortion Habitual medicine.medical_specialty Ceramide Apoptosis Glycerophospholipids Preeclampsia chemistry.chemical_compound Syncytiotrophoblast Pre-Eclampsia Pregnancy Internal medicine medicine Humans Blood Coagulation Phosphatidylethanolamine Sphingolipids Chemistry Immunity Obstetrics and Gynecology Phosphatidic acid Phosphatidylserine medicine.disease Lipids Sphingolipid Trophoblasts Oxidative Stress Cholesterol medicine.anatomical_structure Endocrinology Reproductive Medicine Immunology Female lipids (amino acids peptides and proteins) Cholesterol Esters Sphingomyelin Developmental Biology |
| Zdroj: | Placenta. 34:436-442 |
| ISSN: | 0143-4004 |
| DOI: | 10.1016/j.placenta.2013.02.004 |
| Popis: | Syncytiotrophoblast microvesicles (STBM) are shed from placenta into the maternal circulation. STBM circulate in increased amounts in adverse pregnancies, e.g., preeclampsia and recurrent miscarriages (RM). Recently dysregulation of lipid metabolites has been proposed to be associated with their pathogenesis. Lipid composition of STBM in healthy and adverse pregnancies remains unknown.To determine lipid composition of STBM and whether STBM lipid composition differs in pathologic and normal pregnancies.Patients with Preeclampsia (n = 6) or history of RM (n = 9) (2 consecutive losses20 weeks) and gestational age-matched normal pregnant controls (same number as cases) were recruited. STBM were prepared from placental explant culture supernatant. Lipid profiling of STBM was performed by mass spectrometry in combination with liquid chromatography. We quantified ∼200 lipids in STBM including (i) glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG; phosphatidylserine, PS; phosphatidic acid, PA); (ii) sphingolipids (sphingomyelin, SM; ceramide, Cer; Glucosylceramide, GluCer; ganglioside mannoside 3, GM3); (iii) free cholesterol and cholesteryl esters, CE.The major lipid classes in STBM were SM, Chol, PS, PC and PI, along with PA and GM3 enrichments. SM/PC ratio showed a unique reversal (3:1) compared to that normally found in human cells or plasma. Level of total PS was significantly upregulated (p0.005) in preeclampsia patients, while PI (p0.0005), PA (p0.005), and GM3 (p0.05) were significantly downregulated. Similar trends were obtained in RM.Differential lipid expression of STBM in preeclampsia or RM includes those that are implicated in immune response, coagulation, oxidative stress, and apoptosis. |
| Databáze: | OpenAIRE |
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