Overexpression of VlPRX21 and VlPRX35 genes in Arabidopsis plants leads to bioconversion of trans-resveratrol to δ-viniferin
| Autor: | Sung-Chul Park, Jang Won Pyun, Su Hyun Park, Jae Cheol Jeong, So-Young Kim, Cha Young Kim, Yun-Hee Kim, Yu Jeong Jeong, Jong Rok Lee |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agroinfiltration Physiology Arabidopsis Nicotiana benthamiana Viniferin Plant Science Resveratrol 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Stilbenes Genetics Vitis Benzofurans chemistry.chemical_classification Methyl jasmonate biology Chemistry Phytoalexin food and beverages Resorcinols biology.organism_classification 030104 developmental biology Biochemistry biology.protein 010606 plant biology & botany Peroxidase |
| Zdroj: | Plant Physiology and Biochemistry. 162:556-563 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2021.03.015 |
| Popis: | Stilbenes, including resveratrol and viniferins, a small family of polyphenols, are considered the most important phytoalexin group in Vitis species. In a previous study, we found that co-treatment of methyl jasmonate (MJ) and stevioside (STE) resulted in enhanced extracellular production of viniferins in grapevine cell suspension cultures. Thus, to further understand the mechanisms of viniferin production in grapevine cell cultures, we performed transcriptome analysis and isolated seven candidates of grapevine peroxidase genes (VlAPX6, VlGPX5, VlPRX13, VlPRX21, VlPRX35, VlPRX40, and VlPRX50). Bioconversion of trans-resveratrol to δ-viniferin was examined using crude protein extracts isolated from agroinfiltration-based transient expression of VlPRXs in Nicotiana benthamiana. In addition, we found that crude protein extracts from VlPRX21-, VlPRX35-, and VlPRX40-overexpressing (OX) transgenic Arabidopsis plants led to the conversion of trans-resveratrol to δ-viniferin. We found that in vitro experiments with crude protein extracts from VlPRX21-OX and VlPRX35-OX Arabidopsis plants catalyzed the dimerization of trans-resveratrol to δ-viniferin. Our results suggest that VlPRX21 and VlPRX35 encode functional grapevine class III peroxidases and catalyze the oxidative dimerization of trans-resveratrol to form δ-viniferin in grapevine. |
| Databáze: | OpenAIRE |
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