Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
| Autor: | Ricardo I.F. Trindade, Federico D. Brown, Christopher J. Coates, Juliana de Moraes Leme, Flavia Ariany Belato, Bruno Santos Bezerra, Beatriz Mello, Elisa M. Costa-Paiva |
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| Rok vydání: | 2021 |
| Předmět: |
Cellular respiration
Evolution Molecular biology Science Protein subunit Cell Respiration Citric Acid Cycle Oxidative phosphorylation Biology Article Transcriptome Evolution Molecular Gene duplication Animals Coevolution Phylogeny chemistry.chemical_classification Multidisciplinary Eukaryota NAD Isocitrate Dehydrogenase METABOLISMO CELULAR Isocitrate dehydrogenase Enzyme chemistry Evolutionary biology Medicine NADP |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| Popis: | The biological toolkits for aerobic respiration were critical for the rise and diversification of early animals. Aerobic life forms generate ATP through the oxidation of organic molecules in a process known as Krebs’ Cycle, where the enzyme isocitrate dehydrogenase (IDH) regulates the cycle's turnover rate. Evolutionary reconstructions and molecular dating of proteins related to oxidative metabolism, such as IDH, can therefore provide an estimate of when the diversification of major taxa occurred, and their coevolution with the oxidative state of oceans and atmosphere. To establish the evolutionary history and divergence time of NAD-dependent IDH, we examined transcriptomic data from 195 eukaryotes (mostly animals). We demonstrate that two duplication events occurred in the evolutionary history of NAD-IDH, one in the ancestor of eukaryotes approximately at 1967 Ma, and another at 1629 Ma, both in the Paleoproterozoic Era. Moreover, NAD-IDH regulatory subunits β and γ are exclusive to metazoans, arising in the Mesoproterozoic. Our results therefore support the concept of an ‘‘earlier-than-Tonian’’ diversification of eukaryotes and the pre-Cryogenian emergence of a metazoan IDH enzyme. |
| Databáze: | OpenAIRE |
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