Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development
| Autor: | Vladimir Shulaev, Brett S. Phinney, Richard A. Eigenheer, Ehud Katz, Ho-Youn Kim, Eduardo Blumwald, Florence Negre-Zakharov, Kyung Hwan Boo, Avi Sadka |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Crop and Pasture Production
Proteomics Citrus Sucrose Physiology Phosphatase Plant Biology & Botany Plant Biology Plant Science juice sac cells Biology chemistry.chemical_compound Gene Expression Regulation Plant Tandem Mass Spectrometry Protein biosynthesis Genetics LC-MS/MS Sugar Shotgun proteomics Nutrition Plant Proteins chemistry.chemical_classification food and beverages Plant Research Papers Phosphoric Monoester Hydrolases Amino acid Invertase Emerging Infectious Diseases Infectious Diseases chemistry Biochemistry Gene Expression Regulation Glucosyltransferases Fruit fruit development |
| Zdroj: | Journal of Experimental Botany Journal of experimental botany, vol 62, iss 15 |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits. Our results suggested the occurrence of a metabolic change during citrus fruit maturation, where the organic acid and amino acid accumulation seen during the early stages of development shifted into sugar synthesis during the later stage of citrus fruit development. The expression of invertases remained unchanged, while an invertase inhibitor was up-regulated towards maturation. The increased expression of sucrose-phosphate synthase and sucrose-6-phosphate phosphatase and the rapid sugar accumulation suggest that sucrose is also being synthesized in citrus juice sac cells during the later stage of fruit development. |
| Databáze: | OpenAIRE |
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