Impact of magnetic nanoparticle surface coating on their long-term intracellular biodegradation in stem cells
| Autor: | Aurore Van de Walle, Laurence Motte, Yoann Lalatonne, Rémi Le Borgne, Anouchka Plan Sangnier, Claire Wilhelm, Alberto Curcio |
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| Přispěvatelé: | CNRS UMR 7057 - Laboratoire Matières et Systèmes Complexes (MSC) (MSC), Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC (UMR_7057)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Sorbonne Paris Cité (USPC)-Institut Galilée-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Carboxylic acid media_common.quotation_subject Nanoparticle 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Coated Materials Biocompatible Spheroids Cellular Humans [CHIM]Chemical Sciences General Materials Science [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics Internalization Magnetite Nanoparticles ComputingMilieux_MISCELLANEOUS media_common chemistry.chemical_classification Polyacrylic acid Mesenchymal Stem Cells [CHIM.MATE]Chemical Sciences/Material chemistry 021001 nanoscience & nanotechnology 3. Good health 0104 chemical sciences Surface coating chemistry Biophysics Magnetic nanoparticles Stem cell 0210 nano-technology Intracellular |
| Zdroj: | Nanoscale Nanoscale, Royal Society of Chemistry, 2019, 11 (35), pp.16488-16498. ⟨10.1039/C9NR05624F⟩ |
| ISSN: | 2040-3364 2040-3372 |
| DOI: | 10.1039/C9NR05624F⟩ |
| Popis: | Magnetic nanoparticles (MNPs) internalized within stem cells have paved the way for remote magnetic cell manipulation and imaging in regenerative medicine. A full understanding of their interactions with stem cells and of their fate in the intracellular environment is then required, in particular with respect to their surface coatings. Here, we investigated the biological interactions of MNPs composed of an identical magnetic core but coated with different molecules: phosphonoacetic acid, polyethylene glycol phosphonic carboxylic acid, caffeic acid, citric acid, and polyacrylic acid. These coatings vary in the nature of the chelating function, the number of binding sites, and the presence or absence of a polymer. The nanoparticle magnetism was systematically used as an indicator of their internalization within human stem cells and of their structural long-term biodegradation in a 3D stem cell spheroid model. Overall, we evidence that the coating impacts the aggregation status of the nanoparticles and subsequently their uptake within stem cells, but it has little effect on their intracellular degradation. Only a high number of chelating functions (polyacrylic acid) had a significant protective effect. Interestingly, when the nanoparticles aggregated prior to cellular internalization, less degradation was also observed. Finally, for all coatings, a robust dose-dependent intracellular degradation rate was demonstrated, with higher doses of internalized nanoparticles leading to a lower degradation extent. |
| Databáze: | OpenAIRE |
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