Detecting the abuse of 19-norsteroids in doping controls: A new gas chromatography coupled to isotope ratio mass spectrometry method for the analysis of 19-norandrosterone and 19-noretiocholanolone
Autor: | Davide Curcio, Loredana Iannella, Francesco Botrè, Xavier de la Torre, Cristiana Colamonici |
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Rok vydání: | 2020 |
Předmět: |
Adult
Male Estranes Pharmaceutical Science Urine Mass spectrometry Androsterone 01 natural sciences Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection medicine Environmental Chemistry Humans Nandrolone 030216 legal & forensic medicine Pregnanetriol Isotope-ratio mass spectrometry 19-Norandrosterone Spectroscopy Detection limit Doping in Sports Chromatography Chemistry 010401 analytical chemistry Middle Aged 0104 chemical sciences Substance Abuse Detection Pregnanediol Female Gas chromatography medicine.drug |
Zdroj: | Drug testing and analysisREFERENCES. 13(4) |
ISSN: | 1942-7611 |
Popis: | The detection of 19-norsteroids abuse in doping controls currently relies on the determination of 19-norandrosterone (19-NA) by gas chromatography-tandem mass spectrometry (GC-MS/MS). An additional confirmatory analysis by gas chromatography coupled to isotope ratio mass spectrometry (GC-C-IRMS) is performed on samples showing 19-NA concentrations between 2.5 and 15 ng/ml and not originated from pregnant female athletes or female treated with 19-norethisterone. 19-Noretiocholanolone (19-NE) is typically produced to a lesser extent as a secondary metabolite. The aim of this work was to improve the GC-C-IRMS confirmation procedure for the detection of 19-norsteroids misuse. Both 19-NA and 19-NE were analyzed as target compounds (TCs), whereas androsterone (A), pregnanediol (PD), and pregnanetriol (PT) were selected as endogenous reference compounds (ERCs). The method was validated and applied to urine samples collected by three male volunteers after the administration of nandrolone-based formulations. Before the instrumental analysis, urine samples ( |
Databáze: | OpenAIRE |
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