PPARγ mediates NSAIDs-induced upregulation of TFF2 expression in gastric epithelial cells

Autor: Y. Fujii, Kyoko Tabei, Takashi Hashimoto, Ayako Koitabashi, Takashi Namatame, K. Hosaka, Hideyuki Hiraishi, Masashi Yoneda, Tadahito Shimada, Akira Terano
Rok vydání: 2004
Předmět:
Transcription
Genetic
Gastric epithelial cell
Indomethacin
Muscle Proteins
Receptors
Cytoplasmic and Nuclear
Biochemistry
Genes
Reporter
Structural Biology
Anilides
Enzyme Inhibitors
Receptor
Aspirin
biology
Reverse Transcriptase Polymerase Chain Reaction
Anti-Inflammatory Agents
Non-Steroidal
Trefoil factor family
Peroxisome
Up-Regulation
Cyclooxygenase
Gene Expression Regulation
Neoplastic
Trefoil Factor-2
Trefoil Factor-3
medicine.drug
Transcriptional Activation
medicine.medical_specialty
Biophysics
Peroxisome proliferator-activated receptor γ
Downregulation and upregulation
Stomach Neoplasms
Cell Line
Tumor
Internal medicine
Genetics
medicine
Humans
RNA
Messenger
Molecular Biology
Reporter gene
Dose-Response Relationship
Drug
Neuropeptides
Mucins
Epithelial Cells
Cell Biology
digestive system diseases
Endocrinology
Cancer research
biology.protein
Peptides
Transcription Factors
Zdroj: FEBS Letters. 558:33-38
ISSN: 0014-5793
Popis: Trefoil factor family (TFF) is a group of peptides that play critical roles in maintaining gastric mucosal integrity. In real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and reporter gene assays, we show that indomethacin and aspirin upregulate TFF2 expression in MKN45 gastric cells. These drugs also activated peroxisome proliferator-activated receptor gamma (PPARgamma) at concentration ranges that increase TFF2 expression, and upregulated TFF2 expression was suppressed by GW9662, a specific inhibitor of PPARgamma. These results suggest that indomethacin and aspirin upregulate gastric expression of TFF2 through activation of PPARgamma. This mechanism may be important in reducing the extent of gastric mucosal injury caused by the administration of non-steroidal anti-inflammatory drugs (NSAIDs).
Databáze: OpenAIRE
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