Determination of Oxaliplatin and Curcumin in Combination via Micellar HPLC and Its Method Validation
| Autor: | Ankita Tiwari, Devasish Bose, Pooja Mishra, Ankit Jain, Sanjay K Jain |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Journal of AOAC INTERNATIONAL. 105:999-1007 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoacint/qsac042 |
| Popis: | Background A micellar-HPLC method was developed for the determination of oxaliplatin (OHP) and curcumin (CUR) employing a C18 column [4.6 × 250 mm, particle size (dp) = 5 µm] and diode array detector. Objective A rapid, cost-effective, environmentally friendly, time-efficient, easy-to-handle, and safe method was developed. Methods The conditions were optimized for the estimation of OHP and CUR: 0.15 M sodium dodecyl sulfate (SDS) in 6% (v/v) pentanol buffered to pH 5.0 with a flow rate of 1.0 mL/min, injection volume of 20 µL, and detection at 325 nm. Different analytical parameters, including linearity, accuracy, precision, robustness, specificity, LOD, and LOQ, were determined in compliance with the International Council on Harmonisation (ICH) guidelines. Results The LOD (S/N = 3) of OHP was 0.004 µg/mL and for CUR it was 0.005 µg/mL. The calibration curves for OHP and CUR were linear over the range 0.015–10 µg/mL (determination coefficient r2 = 0.9999) and 0.015–10 µg/mL (r2 = 0.9994), respectively. Conclusion The drugs were eluted in Highlights A simple, cost-effective, eco-friendly HPLC method was developed to simultaneously estimate oxaliplatin and curcumin. The developed method was validated as per the ICH guidelines. |
| Databáze: | OpenAIRE |
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