Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe
| Autor: | Marco P. Monopoli, Brendan Ffrench, Andrea Zendrini, Raymond L. Stalings, Serena Ducoli, Shane Cheung, Paolo Bergese, Dan Wu, John Nolan, Gonzalo Sampedro, Donal F. O'Shea, Olga Piskareva |
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| Rok vydání: | 2018 |
| Předmět: |
Materials Chemistry2506 Metals and Alloys
Boron Compounds 0301 basic medicine Fluorescence-lifetime imaging microscopy Aza Compounds Cell Line Tumor Exosomes Fluorescent Dyes Humans Infrared Rays Optical Imaging Porphobilinogen Surface-Active Agents Catalysis Electronic Optical and Magnetic Materials Ceramics and Composites Chemistry (all) Surfaces Coatings and Films 2506 Exosome Cell Line Flow cytometry Coatings and Films 03 medical and health sciences Labelling Immunoblot Analysis Amphiphile Electronic Materials Chemistry medicine Optical and Magnetic Materials Tumor medicine.diagnostic_test Chemistry Metals and Alloys General Chemistry Fluorescence Microvesicles Surfaces 030104 developmental biology Biophysics |
| Zdroj: | Chemical Communications. 54:7219-7222 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c8cc02135j |
| Popis: | The recognition of the biological, diagnostic and medical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes. |
| Databáze: | OpenAIRE |
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