Characterization of cellulose degrading bacteria from the larval gut of the white grub beetle Lepidiota mansueta (Coleoptera: Scarabaeidae)
| Autor: | Badal Bhattacharyya, Rajen Baruah, Gautam Handique, Amrita Phukan, Syed Wasifur Rahman, Abu Adil Lutful Haque Baruah |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Microorganism 030106 microbiology Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound Enterobacteriaceae medicine Animals Cellulose Scarabaeidae Larva biology General Medicine biology.organism_classification 16S ribosomal RNA Carboxymethyl cellulose Coleoptera Gastrointestinal Tract 030104 developmental biology chemistry Insect Science Carboxymethylcellulose Sodium Bacteria medicine.drug |
| Zdroj: | Archives of insect biochemistry and physiology. 94(2) |
| ISSN: | 1520-6327 |
| Popis: | The goal of this study is to identify and characterize the cellulose degrading microorganisms in the larval gut of the white grub beetle, Lepidiota mansueta. Thirty bacterial strains were isolated and tested for cellulolytic activity using soluble carboxymethyl cellulose (CMC) degrading assays. Of these strains, five (FGB1, FB2, MB1, MB2, and HB1) degrade cellulose. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The highest cellulolytic index (2.14) was found in FGB1. Partial 16S rDNA sequencing, morphological, and biochemical tests were used to identify and characterize the five isolates, all Citrobacter sp. (Enterobacteriaceae). This study identifies new cellulose degrading microorganisms from the larval gut of L. mansueta. The significance of identifying these strains lies in possible application in cellulose degradation. |
| Databáze: | OpenAIRE |
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