S100A8 induces IL-10 and protects against acute lung injury
| Autor: | Kenneth Hsu, Yuka Hiroshima, Nicodemus Tedla, Yuen Ming Chung, Cristan Herbert, Carolyn L. Geczy, Sharron Chow |
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| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Immunology Acute Lung Injury Blotting Western Anti-Inflammatory Agents Pharmacology Lung injury Biology medicine.disease_cause Dexamethasone Proinflammatory cytokine Mice Glycation medicine Immunology and Allergy Animals Cluster Analysis Calgranulin A Lung PI3K/AKT/mTOR pathway Administration Intranasal Mice Inbred BALB C Reverse Transcriptase Polymerase Chain Reaction Epithelial Cells Immunohistochemistry Interleukin-10 IκBα Interleukin 10 Gene Expression Regulation TLR4 Mutant Proteins Transcriptome Oxidative stress Signal Transduction |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 192(6) |
| ISSN: | 1550-6606 |
| Popis: | S100A8 is considered proinflammatory by activating TLR4 and/or the receptor for advanced glycation end products. The aim was to investigate inflammatory effects of S100A8 in murine lung. S100A8 was administered to BALB/c mice by nasal inhalation and genes induced over a time-course assessed. LPS was introduced intranasally either alone or 2 h after pretreatment of mice with intranasal application of S100A8 or dexamethasone. A Cys42-Ala42 mutant S100A8 mutant was used to assess whether S100A8’s effects were via pathways that were dependent on reactive oxygen species. S100A8 induced IL-10 mRNA, and expression was apparent only in airway epithelial cells. Importantly, it suppressed acute lung injury provoked by LPS inhalation by suppressing mast-cell activation and induction of mediators orchestrating leukocyte recruitment, possibly by reducing NF-κB activation via an IκBα/Akt pathway and by downmodulating pathways generating oxidative stress. The Cys42-Ala42 S100A8 mutant did not induce IL-10 and was less immunosuppressive, indicating modulation by scavenging oxidants. S100A8 inhibition of LPS-mediated injury was as potent, and outcomes were remarkably similar to immunosuppression by dexamethasone. We challenge the notion that S100A8 is an agonist for TLR4 or the receptor for advanced glycation end products. S100A8 induced IL-10 in vivo and initiates a feedback loop that attenuates acute lung injury. |
| Databáze: | OpenAIRE |
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