Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway
| Autor: | Steven J. Feinmark, Michael A. Nardi, Simon Karpatkin, Zongdong Li, Liang Hu |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Blood Platelets
Lysis HIV Infections Arachidonate 12-Lipoxygenase Peroxide Antibodies Article chemistry.chemical_compound Lipoxygenase Humans Platelet Fragmentation (cell biology) chemistry.chemical_classification Reactive oxygen species NADPH oxidase biology Chemistry NADPH Oxidases Complement System Proteins General Medicine Thrombocytopenia Molecular biology Biochemistry HIV-1 biology.protein Reactive Oxygen Species Oxidation-Reduction NADP |
| Zdroj: | Journal of Clinical Investigation. 113:973-980 |
| ISSN: | 0021-9738 |
| Popis: | Antiplatelet GPIIIa49–66 Ab of HIV-related thrombocytopenic patients induces thrombocytopenia and platelet fragmentation by the generation of peroxide and other reactive oxygen species (ROS). Here we report the presence of a functional platelet NADPH oxidase pathway that requires activation by the platelet 12-lipoxygenase (12-LO) pathway to fragment platelets. A new Ab-mediated mechanism is described in which the platelet 12-LO product, 12(S)-HETE activates the NADPH oxidase pathway to generate ROS. |
| Databáze: | OpenAIRE |
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