Hypusinated eIF5A is expressed in the pancreas and spleen of individuals with type 1 and type 2 diabetes

Autor: Raghavendra G. Mirmira, Stephanie C. Colvin, Teresa L. Mastracci, Leah R. Padgett
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Physiology
Immunofluorescence
DHPS
Biochemistry
chemistry.chemical_compound
Mice
White Blood Cells
0302 clinical medicine
Endocrinology
Mice
Inbred NOD
Peptide Initiation Factors
Animal Cells
Insulin-Secreting Cells
Immune Physiology
Medicine and Health Sciences
Deoxyhypusine synthase
NOD mice
Multidisciplinary
biology
T Cells
RNA-Binding Proteins
Animal Models
Middle Aged
Pancreatic Polypeptide-Secreting Cells
3. Good health
Type 2 Diabetes
Experimental Organism Systems
Medicine
Female
Beta cell
Anatomy
Cellular Types
EIF5A
Research Article
Adult
Endocrine Disorders
Science
Immune Cells
Immunology
Endocrine System
Mouse Models
Research and Analysis Methods
03 medical and health sciences
Young Adult
Immune system
Exocrine Glands
Model Organisms
Diabetes Mellitus
Animals
Humans
Immunoassays
Pancreas
Hypusine
Innate immune system
Blood Cells
Lysine
Biology and Life Sciences
Polypeptides
Cell Biology
Molecular biology
Mice
Inbred C57BL
030104 developmental biology
Diabetes Mellitus
Type 1
chemistry
Diabetes Mellitus
Type 2
Metabolic Disorders
biology.protein
Animal Studies
Immunologic Techniques
Peptides
030217 neurology & neurosurgery
Spleen
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 3, p e0230627 (2020)
ISSN: 1932-6203
Popis: The gene encoding eukaryotic initiation factor 5A (EIF5A) is found in diabetes-susceptibility loci in mouse and human. eIF5A is the only protein known to contain hypusine (hydroxyputrescine lysine), a polyamine-derived amino acid formed post-translationally in a reaction catalyzed by deoxyhypusine synthase (DHPS). Previous studies showed pharmacologic blockade of DHPS in type 1 diabetic NOD mice and type 2 diabetic db/db mice improved glucose tolerance and preserved beta cell mass, which suggests that hypusinated eIF5A (eIF5AHyp) may play a role in diabetes pathogenesis by direct action on the beta cells and/or altering the adaptive or innate immune responses. To translate these findings to human, we examined tissue from individuals with and without type 1 and type 2 diabetes to determine the expression of eIF5AHyp. We detected eIF5AHyp in beta cells, exocrine cells and immune cells; however, there was also unexpected enrichment of eIF5AHyp in pancreatic polypeptide-expressing PP cells. Interestingly, the presence of eIF5AHyp co-expressing PP cells was not enhanced with disease. These data identify new aspects of eIF5A biology and highlight the need to examine human tissue to understand disease.
Databáze: OpenAIRE
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