Hypusinated eIF5A is expressed in the pancreas and spleen of individuals with type 1 and type 2 diabetes
Autor: | Raghavendra G. Mirmira, Stephanie C. Colvin, Teresa L. Mastracci, Leah R. Padgett |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Male Physiology Immunofluorescence DHPS Biochemistry chemistry.chemical_compound Mice White Blood Cells 0302 clinical medicine Endocrinology Mice Inbred NOD Peptide Initiation Factors Animal Cells Insulin-Secreting Cells Immune Physiology Medicine and Health Sciences Deoxyhypusine synthase NOD mice Multidisciplinary biology T Cells RNA-Binding Proteins Animal Models Middle Aged Pancreatic Polypeptide-Secreting Cells 3. Good health Type 2 Diabetes Experimental Organism Systems Medicine Female Beta cell Anatomy Cellular Types EIF5A Research Article Adult Endocrine Disorders Science Immune Cells Immunology Endocrine System Mouse Models Research and Analysis Methods 03 medical and health sciences Young Adult Immune system Exocrine Glands Model Organisms Diabetes Mellitus Animals Humans Immunoassays Pancreas Hypusine Innate immune system Blood Cells Lysine Biology and Life Sciences Polypeptides Cell Biology Molecular biology Mice Inbred C57BL 030104 developmental biology Diabetes Mellitus Type 1 chemistry Diabetes Mellitus Type 2 Metabolic Disorders biology.protein Animal Studies Immunologic Techniques Peptides 030217 neurology & neurosurgery Spleen |
Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 3, p e0230627 (2020) |
ISSN: | 1932-6203 |
Popis: | The gene encoding eukaryotic initiation factor 5A (EIF5A) is found in diabetes-susceptibility loci in mouse and human. eIF5A is the only protein known to contain hypusine (hydroxyputrescine lysine), a polyamine-derived amino acid formed post-translationally in a reaction catalyzed by deoxyhypusine synthase (DHPS). Previous studies showed pharmacologic blockade of DHPS in type 1 diabetic NOD mice and type 2 diabetic db/db mice improved glucose tolerance and preserved beta cell mass, which suggests that hypusinated eIF5A (eIF5AHyp) may play a role in diabetes pathogenesis by direct action on the beta cells and/or altering the adaptive or innate immune responses. To translate these findings to human, we examined tissue from individuals with and without type 1 and type 2 diabetes to determine the expression of eIF5AHyp. We detected eIF5AHyp in beta cells, exocrine cells and immune cells; however, there was also unexpected enrichment of eIF5AHyp in pancreatic polypeptide-expressing PP cells. Interestingly, the presence of eIF5AHyp co-expressing PP cells was not enhanced with disease. These data identify new aspects of eIF5A biology and highlight the need to examine human tissue to understand disease. |
Databáze: | OpenAIRE |
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