Spinal cord stimulation induces c-Fos expression in the dorsal horn in rats with neuropathic pain after partial sciatic nerve injury
| Autor: | Helwin Smits, Maarten van Kleef, Philipp Gobrecht, Wiel Honig, Elbert A.J. Joosten, Job Gerver |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Gene Expression Electric Stimulation Therapy c-Fos Rats Sprague-Dawley Physical Stimulation medicine Animals Nociception assay integumentary system biology Horn (anatomy) business.industry General Neuroscience Histology Anatomy Sciatic nerve injury medicine.disease Sciatic Nerve Electrodes Implanted Rats Posterior Horn Cells Electrophysiology Allodynia nervous system Neuropathic pain biology.protein Neuralgia medicine.symptom business Proto-Oncogene Proteins c-fos tissues |
| Zdroj: | Neuroscience Letters. 450:70-73 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2008.11.013 |
| Popis: | Spinal cord stimulation (SCS) is an established treatment for intractable neuropathic pain, especially CRPS-1. The mechanisms of action of SCS have only been partly elucidated and include suppression of the hyper-excitability of the Wide Dynamic Range neurons and a GABA increase in the dorsal horn. In the present study we demonstrate an increase of c-Fos immunoreactive cells in the dorsal horn after SCS, suggesting early cellular activation that may preclude earlier described electrophysiological and biochemical changes in the dorsal horn after SCS. In a rat model of neuropathic pain, allodynia was induced and quantified using the von Frey test. In 11 rats a SCS device was implanted and spinal cord stimulation performed. Withdrawal threshold were measured every 15 min up to 90 min. A sham group (n=6) also had a SCS device implanted, but did not receive SCS. After SCS the animals were perfused and histology was performed for quantification of c-Fos immunoreactivity in the dorsal horns. We found a significant increase in c-Fos in the SCS group compared to our sham group and control tissue, indicating late cellular activity in the dorsal horn after SCS. |
| Databáze: | OpenAIRE |
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