Effect of refrigeration at -1°C on spermatozoa quality of domestic cats
| Autor: | Josiana de Fátima Schnitzer, Maria Isabel Mello Martins, Anne Kemmer Souza, Felipe Montanheiro Perencin, Cristiane Sella Paranzini, Luiz Guilherme Corsi Trautwein, Guilherme Schiess Cardoso |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
Veterinary medicine gatos domésticos eletroejaculação cryopreservation Electroejaculation Cryopreservation law.invention 0403 veterinary science Animal science law Refrigeration spermatozoa domestic cats SF600-1100 medicine coconut water extender epidídimo CATS General Veterinary Chemistry criopreservação Extender cats 0402 animal and dairy science Vas deferens diluente de água de coco 04 agricultural and veterinary sciences Epididymis 040201 dairy & animal science Sperm medicine.anatomical_structure Refrigeração electroejaculation espermatozoides epididymis |
| Zdroj: | Pesquisa Veterinária Brasileira v.40 n.4 2020 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Volume: 40, Issue: 4, Pages: 306-314, Published: 17 JUN 2020 Pesquisa Veterinária Brasileira, Vol 40, Iss 4, Pp 306-314 (2020) |
| Popis: | The objective of this study was to evaluate the sperm quality obtained of domestic cats by electroejaculation and recovery of the tail of the epididymis after cooling at -1°C and 4°C for 24 and 48 hours. Twenty-nine adult cats (2 to 6kg) were used. Sperm collection was performed by electroejaculation (EEJ), and after 48 hours, the cats were orchiectomized, and sperm sample was obtained from the vas deferens and epididymis tail (EPD). The samples were diluted in ACP-117® extender, and the sperm characteristics were evaluated at three different moments: when still fresh, 24 and 48 hours after cooling. In order to compare the two refrigeration temperatures, the first stage was to analyze if there was a difference between the harvesting techniques. After this, two experiments were conducted: in the first, sperm sample from 14 cats were used and the cooling was performed at -1°C; and in the second, sample from 15 cats were used and the sperm were refrigerated at 4°C. Sperm kinetics were evaluated by computerized analysis (CASA) and concentration by Neubauer chamber, spermatic morphology was evaluated by modified Karras staining, and membrane integrity was evaluated by eosin nigrosine. The results obtained were analyzed in R software, version 3.2.5 using the Mann-Whitney test for variables with abnormal distributions, considering significance at the level of 5%. In ejaculate samples, higher values of total morphological defects were observed after 24 and 48 hours of refrigeration at 4°C (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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