Procedure for selecting monoclonal antibodies for use in a ligand displacement assay of serum antibody levels
| Autor: | Patricia G. Voss, B.L. Middlebrooks, Paul M. Toom, Williard L. Douglas |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.drug_class
Trichinella Immunology Antibodies Helminth Antibody Affinity Enzyme-Linked Immunosorbent Assay Monoclonal antibody Ligands Binding Competitive Antibodies Mice Antigen Antibody Specificity medicine Animals Avidity Pharmacology Antiserum chemistry.chemical_classification Immunoassay Hybridomas biology Antibodies Monoclonal Trichinellosis Ligand (biochemistry) Molecular biology Enzyme chemistry biology.protein Rabbits Antibody Conjugate |
| Zdroj: | Journal of immunoassay. 12(1) |
| ISSN: | 0197-1522 |
| Popis: | Monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. A screening process is described which involves measurement of displacement of antibody by an ELISA procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody in a ligand displacement format without necessitating the production of enzyme conjugates of each candidate antibody to be screened. The procedure was used to screen a set of eleven monoclonal antibodies (initially selected for anti-Trichinella spiralis specificity by conventional screening methods), and successfully discriminated between antibodies which were useful in the ligand displacement formet and those which were not. |
| Databáze: | OpenAIRE |
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