Physisorption and chemisorption of T4 bacteriophages on amino functionalized silica particles
| Autor: | Edo Bar-Zeev, Stephanie Bone, Jasmina Markovski, Morteza Abbaszadegan, Kiril Hristovski, François Perreault, Yair Kaufman, Absar Alum |
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| Rok vydání: | 2018 |
| Předmět: |
Surface Properties
viruses Static Electricity 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Adsorption Physisorption Bacteriophage T4 Amines Particle Size Carbodiimide Chemistry Sorption Adhesion Silicon Dioxide 021001 nanoscience & nanotechnology Microspheres 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Carbodiimides Cross-Linking Reagents Chemical engineering Chemisorption Particle Surface modification 0210 nano-technology |
| Zdroj: | Journal of Colloid and Interface Science. 532:68-76 |
| ISSN: | 0021-9797 |
| Popis: | Bacteriophages, or phages, are receiving increasing interest as recognition tools for the design of bioactive surfaces. However, to maintain the activity of surface-bound phages, the immobilization strategy must provide the right orientation and not compromise the phages’ integrity. The objectives of this study were to characterize the phage sorption capacity and the immobilized phage activity for aminated silica particles functionalized with T4 phages. Two functionalization strategies were compared; physisorption, based on electrostatic adhesion, and chemisorption, where the phage and the particle are coupled using a carbodiimide cross-linker. We report that chemisorption, at maximum adsorption conditions on 1 µm particles, yielded 16 functional phages per particle, which is 2.5 times more than by the physisorption method. Particle diameter is shown to have an important impact on phage attachment and 1.8 µm particles were found to have ∼4 times more phages per surface area than 0.5 µm particles. Higher surface coverage is attributed to the lower steric hindrance on bigger particles. These findings provide important guidelines for the design of phage-functionalized particles for environmental, biomedical, or sensing applications. |
| Databáze: | OpenAIRE |
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