Studies on photosynthetic phosphorylation III. Relation between photosynthetic phosphorylation and reduction of triphosphopyridine nucleotide by chloroplasts

Autor: J.S.C. Wessels
Rok vydání: 1959
Předmět:
Zdroj: Biochimica et Biophysica Acta. 35:53-64
ISSN: 0006-3002
DOI: 10.1016/0006-3002(59)90334-8
Popis: The photochemical reduction of TPN by isolated chloroplasts was investigated. A comparison of the rate of TPN reduction with that of photosynthetic phosphorylation provided evidence that the generation of ATP in the presence of vitamin K 3 or FMN is not coupled with the reoxidation of TPNH by the oxidized product of the photolysis of water. Photosynthetic phosphorylation could proceed unimpaired under conditions in which the chloroplasts had lost their ability to reduce TPN. On the other hand, TPN reduction could be considerably stimulated by a chloroplast extract which did not affect photosynthetic phosphorylation. These results are discussed in relation to the recent finding that the reduction of TPN by chloroplasts is accompanied by ATP formation.
Databáze: OpenAIRE