Topical interleukin-1 receptor antagonist inhibits inflammatory cell infiltration into the cornea
| Autor: | Shyam S. Chaurasia, Rajiv R. Mohan, W. Michael Stapleton, Steven E. Wilson, Fabricio Witzel de Medeiros, Sunilima Sinha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell medicine.drug_class Corneal Stroma Anti-Inflammatory Agents Drug Evaluation Preclinical Inflammation Bone Marrow Cells Pharmacology Biology Article Monocytes Cornea Cellular and Molecular Neuroscience Leukocyte Count Mice Stroma Cell Movement medicine Animals Receptor Wound Healing CD11b Antigen Epithelium Corneal Interleukin Receptors Interleukin-1 Receptor antagonist Sensory Systems Recombinant Proteins Mice Inbred C57BL Ophthalmology Disease Models Animal Interleukin 1 Receptor Antagonist Protein medicine.anatomical_structure Interleukin 1 receptor antagonist Female medicine.symptom Ophthalmic Solutions |
| Popis: | Interleukin (IL)-1alpha and beta are important modulators of many functions of corneal epithelial and stromal cells that occur following injury to the cornea, including the influx of bone marrow-derived inflammatory cells into the stroma attracted by chemokines released from the stroma and epithelium. In this study, we examined the effect of topical soluble IL-1 receptor antagonist on bone marrow-derived cell influx following corneal epithelial scrape injury in a mouse model. C57BL/6 mice underwent corneal epithelial scrape followed by application of IL-1 receptor antagonist (Amgen, Thousand Oaks, CA) at a concentration of 20 mg/ml or vehicle for 24 h prior to immunocytochemical detection of marker CD11b-positive cells into the stroma. In two experiments, topical IL-1 receptor antagonist had a marked effect in blocking cell influx. For example, in experiment 1, topical IL-1 receptor antagonist markedly reduced detectible CD11b-positive cells into the corneal stroma at 24h after epithelial injury compared with the vehicle control (3.5+/-0.5 (standard error of the mean) cells/400x field and 13.9+/-1.2 cells/400x field, respectively, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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