Tissue specificity of mitochondrial adaptations in rats after 4 weeks of normobaric hypoxia

Autor: Giuseppe Miserocchi, Alice Panariti, Gaia Buoli Comani, Ilaria Rivolta, Marcella Rocchetti, David Bishop, Alessandra Ferri
Přispěvatelé: Ferri, A, Panariti, A, Miserocchi, G, Rocchetti, M, Buoli Comani, G, Rivolta, I, Bishop, D
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Physiology
Cell Respiration
Citrate (si)-Synthase
Oxidative phosphorylation
Mitochondria
Heart
03 medical and health sciences
BIO/09 - FISIOLOGIA
Ventricular hypertrophy
Physiology (medical)
Internal medicine
Respiration
medicine
Animals
Citrate synthase
Orthopedics and Sports Medicine
Skeletal muscles
Rats
Wistar
Muscle
Skeletal
Hypoxia
Organelle Biogenesis
biology
Chemistry
Myocardium
Public Health
Environmental and Occupational Health
Heart
General Medicine
Hypoxia (medical)
medicine.disease
Adaptation
Physiological
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Rats
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Mitochondrial biogenesis
Organ Specificity
Ventricle
biology.protein
medicine.symptom
Homeostasis
Mitochondrial respiration
Zdroj: European Journal of Applied Physiology. 118:1641-1652
ISSN: 1439-6327
1439-6319
DOI: 10.1007/s00421-018-3897-9
Popis: Purpose: Exposure to hypoxia has been suggested to activate multiple adaptive pathways so that muscles are better able to maintain cellular energy homeostasis. However, there is limited research regarding the tissue specificity of this response. The aim of this study was to investigate the influence of tissue specificity on mitochondrial adaptations of rat skeletal and heart muscles after 4 weeks of normobaric hypoxia (FiO2: 0.10). Methods: Twenty male Wistar rats were randomly assigned to either normobaric hypoxia or normoxia. Mitochondrial respiration was determined in permeabilised muscle fibres from left and right ventricles, soleus and extensorum digitorum longus (EDL). Citrate synthase activity and the relative abundance of proteins associated with mitochondrial biogenesis were also analysed. Results: After hypoxia exposure, only the soleus and left ventricle (both predominantly oxidative) presented a greater maximal mass-specific respiration (+48 and +25%, p < 0.05) and mitochondrial-specific respiration (+75 and +28%, p < 0.05). Citrate synthase activity was higher in the EDL (0.63 ± 0.08 vs 0.41 ± 0.10 µmol min− 1 µg− 1) and lower in the soleus (0.65 ± 0.17 vs 0.87 ± 0.20 µmol min− 1 µg− 1) in hypoxia with respect to normoxia. There was a lower relative protein abundance of PGC-1α (−25%, p < 0.05) in the right ventricle and a higher relative protein abundance of PGC-1β (+43%, p < 0.05) in the left ventricle of rats exposed to hypoxia, with few differences for protein abundance in the other muscles. Conclusion: Our results show a muscle-specific response to 4 weeks of normobaric hypoxia. Depending on fibre type, and the presence of ventricular hypertrophy, muscles respond differently to the same degree of environmental hypoxia
Databáze: OpenAIRE
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