UPLC-MS Metabolome-Based Seed Classification of 16 Vicia Species: A Prospect for Phyto-Equivalency and Chemotaxonomy of Different Accessions
| Autor: | Mohamed A. Farag, Nesrin M Fayek, Reham Hassan Mekky, Matthias Kropf, Clarice Noleto Dias, Ludger A. Wessjohann, Andreas G. Heiss |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
01 natural sciences Metabolomics Tandem Mass Spectrometry Botany Metabolome Vicia sepium Chromatography High Pressure Liquid Flavonoids biology fungi 010401 analytical chemistry food and beverages General Chemistry biology.organism_classification Terpenoid 0104 chemical sciences Vicia faba Vicia Chemotaxonomy Seeds Vicia hirsuta General Agricultural and Biological Sciences Chromatography Liquid 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:5252-5266 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Seeds of domesticated Vicia (vetch) species (family Fabaceae-Faboideae) are produced and consumed worldwide for their nutritional value. Seed accessions belonging to 16 different species of Vicia-both domesticated and wild taxa-were subjected to a chemotaxonomic study using ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) analyzed by chemometrics. A total of 89 metabolites were observed in the examined Vicia accessions. Seventy-eight out of the 89 detected metabolites were annotated. Metabolites quantified belonged to several classes, viz., flavonoids, procyanidins, prodelphinidins, anthocyanins, stilbenes, dihydrochalcones, phenolic acids, coumarins, alkaloids, jasmonates, fatty acids, terpenoids, and cyanogenics, with flavonoids and fatty acids amounting to the major classes. Flavonoids, fatty acids, and anthocyanins showed up as potential chemotaxonomic markers in Vicia species discrimination. Fatty acids were more enriched in Vicia faba specimens, while the abundance of flavonoids was the highest in Vicia parviflora. Anthocyanins allowed for discrimination between Vicia hirsuta and Vicia sepium. To the best of our knowledge, this is the first report on employing UPLC-MS metabolomics to discern the diversity of metabolites at the intrageneric level among Vicia species. |
| Databáze: | OpenAIRE |
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